Purified anti-human IL-6 Maxpar Ready Antibody anti-IL-6

Clone: MQ2-13A5 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype: Rat IgG1, κ
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Product Citations: publications

MQ2-13A5_Purified_IL-6_CyTOF_1_112113 — Human PBMCs were incubated for 18 hours in media alone (bottom) or with LPS (top) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 162Dy-anti-CD69 and 47Sm-anti-IL-6 (MQ2-13A5). Data provided by DVS Sciences.

Cat #	Size	Price	Save
501115	100 µg	¥31,020

Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: COS-7- expressed, recombinant human IL-6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: ELISA or ELISPOT Capture^1-3,7: The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.

Neutralization^1-3,5,6: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Additional applications (for the relevant formats) include: intracellular flow cytometry¹⁰.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2562841 (BioLegend Cat. No. 501115)

Antigen Details

Structure: Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity: Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources: T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets: T cells, B cells, hepatocytes, cholinergic neurons
Receptors: Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Int. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. van Oers M, et al. 1993. Ann. Hematol. 66:219.
Regulation: Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, and IL-17; downregulated by glucocorticoids IL-4 and TGF-β
Gene ID: 3569 View all products for this Gene ID
UniProt: View information about IL-6 on UniProt.org

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All IL-6 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human IL-6	MQ2-13A5	ICFC
PE anti-human IL-6	MQ2-13A5	ICFC
Purified anti-human IL-6	MQ2-13A5	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
APC anti-human IL-6	MQ2-13A5	ICFC
Pacific Blue™ anti-human IL-6	MQ2-13A5	ICFC
Purified anti-human IL-6 (Maxpar® Ready)	MQ2-13A5	ELISA Capture,CyTOF®
PerCP/Cyanine5.5 anti-human IL-6	MQ2-13A5	ICFC
PE/Cyanine7 anti-human IL-6	MQ2-13A5	ICFC
Alexa Fluor® 647 anti-human IL-6	MQ2-13A5	ICFC
PE/Dazzle™ 594 anti-human IL-6	MQ2-13A5	ICFC
Ultra-LEAF™ Purified anti-human IL-6	MQ2-13A5	ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
TotalSeq™-B0942 anti-human IL-6	MQ2-13A5	ICPG

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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