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- Clone
- 7H20-I19-M3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin 21
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant Human IL-21 (lane 1) and Mouse IL-21 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-HU/MU IL-21 antibody (clone 7H20-I19-M3). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminscence detection.
Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length human IL-21
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store frozen between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product weakly reacts with Mouse.
- Product Citations
-
- RRID
-
AB_1227666 (BioLegend Cat. No. 514302)
Antigen Details
- Distribution
-
Th17
- Function
- Immunomodulation
- Cell Sources
- NK-T cells and T cells
- Cell Targets
- cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
- Receptors
- IL-21R
- Cell Type
- Th17
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Nurieva R,et al. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57. - Gene ID
- 59067 View all products for this Gene ID 60505 View all products for this Gene ID
- UniProt
- View information about IL-21 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All IL-21 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human/mouse IL-21 | 7H20-I19-M3 | WB |
| Biotin anti-human/mouse IL-21 | 7H20-I19-M3 | WB,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human/mouse IL-21
Recombinant Human IL-21 (lane 1) and Mouse IL-21 (lane 2) we... -
Biotin anti-human/mouse IL-21
Recombinant Human IL-21 (lane 1) and Mouse IL-21 (lane 2) we... 
Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and trea...
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