- Clone
- MHN3-21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neurogenic locus notch homolog protein 3, Notch-3, CADASIL
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The Notch receptors are highly conserved from invertebrates to mammals. While Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch3 gene mutation can cause CADASIL, a hereditary early stroke syndrome.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Notch 3-human IgG1 Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
- RRID
-
AB_2563786 (BioLegend Cat. No. 345407)
Antigen Details
- Structure
- Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
- Cell Type
- Tregs
- Biology Area
- Immunology
- Antigen References
-
1. Ehebauer ME. et al. 2006. Biochem J 392:13
2. Joutel A et al. 1996. Nature 383:707
3. Shimizu K et al. 2000. Mol Cell Biology 20:18
4. Tanigaki K et al. 2007. Nature Immunol 8:451
5. Bellavia D et al. 2008. 27:5092
6. Louvi A et al. Dev Neurosci. 2006. 28:5 - Gene ID
- 4854 View all products for this Gene ID
- UniProt
- View information about Notch 3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All Notch 3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Notch 3 | MHN3-21 | FC |
PE anti-human Notch 3 | MHN3-21 | FC |
Purified anti-human Notch 3 (Maxpar® Ready) | MHN3-21 | FC,CyTOF® |
APC anti-human Notch 3 | MHN3-21 | FC |
TotalSeq™-A0233 anti-human Notch 3 | MHN3-21 | PG |
TotalSeq™-B0233 anti-human Notch 3 | MHN3-21 | PG |
TotalSeq™-C0233 anti-human Notch 3 | MHN3-21 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Notch 3
Human T cell leukemia cell line HPB-ALL stained with MHN3-21... -
PE anti-human Notch 3
Human T cell leukemia cell line HPB-ALL stained with MHN3-21... -
Purified anti-human Notch 3 (Maxpar® Ready)
Human Jurkat T cells (top) and human NALM-6 pre-B cells (bot... -
APC anti-human Notch 3
HPB-ALL, human T cell leukemia cell line was stained with No... -
TotalSeq™-A0233 anti-human Notch 3
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TotalSeq™-B0233 anti-human Notch 3
-
TotalSeq™-C0233 anti-human Notch 3
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