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Purified anti-human ROR1 Antibody

Pricing & Availability
Clone
2A2 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine kinase-like orphan receptor 1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase receptor-related 1, NTRKR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2A2_Purified_ROR1_Antibody_FC_040113
Human teratocarcinoma cell line NCCIT was stained with purified ROR1 (clone 2A2, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 2A2_Purified_ROR1_Antibody_FC_040113
    Human teratocarcinoma cell line NCCIT was stained with purified ROR1 (clone 2A2, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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Cat # Size Price Save
357802 100 µg ¥50,380
Description

ROR1, also known as NTRKR1, is a type I transmembrane protein and member of the ROR subfamily of surface receptors. ROR1 consists of one frizzled domain, one Ig-like C2-type domain, one kringle domain, and one kinase domain with no catalytic activity. ROR1 is expressed on embryonic tissue, in the central nervous system and on some cancer cells, and is used as a marker for B-cell chronic lymphocytic leukemia. Wnt5a has been identified as a ligand for ROR1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.

Application References

(PubMed link indicates BioLegend citation)
  1. Dave H, et al. 2012. PLoS One 7:e52655. (FC)
  2. Baskar S, et al. 2012. MAbs. 4:349. (FC)
RRID
AB_2562006 (BioLegend Cat. No. 357802)

Antigen Details

Structure
Type I transmembrane protein, consist of 1 frizzled domain, 1 Ig-like C2-type domain, 1 kringle domain, and 1 non-functional kinase domain; member of the ROR subfamily of surface receptors
Distribution

Embryonic tissue, central nervous system, chronic lymphocytic leukemia cells

Ligand/Receptor
Wnt5a
Cell Type
Embryonic Stem Cells, Leukemia
Biology Area
Immunology, Stem Cells
Antigen References
  1. Bicocca VT, et al. 2012. Cancer Cell. 22:656.
  2. Zhang S, et al. 2012. PLoS One 7:e31127.
  3. Uhrmacher S, et al. 2011. Leuk Res. 35:1360.
  4. Yang J, et al. 2011. PLoS One 6:e21018.
  5. Grumolato L, et al. 2010. Genes Dev. 24:2517.
Gene ID
4919 View all products for this Gene ID
UniProt
View information about ROR1 on UniProt.org
Go To Top Version: 2    Revision Date: 07/17/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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