Purified anti-human TRA-2-49 Antibody anti-TRA-2-49

Clone: TRA-2-49/6E (See other available formats)
Regulatory Status: RUO
Other Names: Liver/Bone/Kidney Alkaline Phosphatase
Isotype: Mouse IgG1, κ
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Product Citations: publications

TRA-2-496E_Purified_TRA-2-49_Antibody_FC_070313 — Human teratocarcinoma cell line NCCIT cells were stained with purified TRA-2-49 (clone TRA-2-49/6E) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.

Cat #	Size	Price	Save
358702	100 µg	¥73,700

Description

TRA-2-49, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone, and kidney cells. It has been reported that the expression level of TRA-2-49 is decreased upon differentiation; therefore measurement of TRA-2-49 expression can be used to monitor the differentiation status of pluripotent stem cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human embryonal carcinoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats) include: immunohistochemistry¹ and immunofluorescence¹.
RRID: AB_2562261 (BioLegend Cat. No. 358702)

Antigen Details

Distribution: Undifferentiated embryonic stem (ES) cells, embryonal carcinoma (EC) cells, most EC cell lines, bone, liver, kidney
Cell Type: Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Andrews PW, et al. 1996. Int. J. Cancer. 66:806.
2. Yan X, et al. 2010. Stem Cells Dev. 19:469. (ICC)
3. Zou XY, et al. 2012. Stem Cell Res. Ther. 3:43. (ICC)
Gene ID: 249 View all products for this Gene ID
UniProt: View information about TRA-2-49 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All TRA-2-49 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TRA-2-49	TRA-2-49/6E	FC,ICC
PE anti-human TRA-2-49	TRA-2-49/6E	FC
Alexa Fluor® 488 anti-human TRA-2-49	TRA-2-49/6E	FC
Alexa Fluor® 647 anti-human TRA-2-49	TRA-2-49/6E	ICC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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