- Clone
- 15B11.3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thymic stromal lymphopoietin (TSLP)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Thymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Recent studies revealed that expression of TSLP has a direct link to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells which trigger inflammatory Th2 response characterized by high TNF-alpha and little IL-10 production.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human TSLP
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng/ml are recommended for each ELISA plate.
- Application Notes
-
ELISA Capture: Purified and Ultra-LEAF™ purified 15B11.3 antibody are useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5159 antibody (Cat. No. 515903) as the detecting antibody.
-
Application References
(PubMed link indicates BioLegend citation) -
- Yeh CY, et al. 2013. PLoS One. 8:e55472. (Neut) PubMed
- RRID
-
AB_940352 (BioLegend Cat. No. 512202)
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Liu YJ. 2007. J. Allergy Clin. Immunol. 120:238.
2. Nagata Y. 2007. Int. Arch. Allergy Immunol. 144:305.
3. Jiang Q, et al. 2007 BMC Immunol. 8:11.
4. Kato A. et al. 2007. J. Immunol. 179:1080.
5. Rochman I, et al. 2007 J. Immunol. 178:6720.
6. Reche PA, et al. 2001. J. Immunol. 167:336.
7. Pandey A, et al. 2000. Nat. Immunol. 1:59.
8. Park LS, et al. 2000. J Exp. Med. 192:659. - Gene ID
- 85480 View all products for this Gene ID
- UniProt
- View information about TSLP on UniProt.org
Related FAQs
Other Formats
View All TSLP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human TSLP | 15B11.3 | ELISA |
Ultra-LEAF™ Purified anti-human TSLP | 15B11.3 | ELISA Capture,Neut |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human TSLP
Human TSLP Direct ELISA (Clone 15B11) -
Ultra-LEAF™ Purified anti-human TSLP
Human TSLP Direct ELISA (Clone 15B11)
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