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Purified anti-IL-36α Antibody

Pricing & Availability
Clone
O91D11 (See other available formats)
Regulatory Status
RUO
Other Names
IL-36 alpha, IL-1F6, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon
Isotype
Mouse IgG1, κ
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Product Citations
publications
O91D11_Purified_IL-36alpha_Antibody_WB_043015
Recombinant human IL-36α (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IL-36α antibody (clone O91D11) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • O91D11_Purified_IL-36alpha_Antibody_WB_043015
    Recombinant human IL-36α (50 ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IL-36α antibody (clone O91D11) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # Size Price Save
676402 100 µg ¥50,380
Description

IL-36α is one of the IL-36 cytokines that are part of the IL-1 family. Like other IL-1 family members, IL-36α requires N-terminal processing to gain more potent bioactivity. Currently, the proteases responsible for processing IL-36α are unknown. IL-36α signals through IL-36R/IL-1RAcP, which results in MAPK, Erk1/2, and JNK activation. IL-36α is implicated in skin homeostasis, and it is overexpressed in psoriatic lesional skin. Transgenic mice overexpressing IL-36α in skin have an inflammatory skin condition, which shows some characteristics of human psoriasis, including thickened scaly skin, acanthosis, hyperkeratosis, and dermis infiltration. EGF regulates the expression of IL-36α in the skin. IL-36α can also be detected in adipose tissue. IL-36α reduces adipocyte differentiation and also induces inflammatory gene expression in mature adipocytes. In the lungs, the expression of IL-36α is increased in response to inflammatory stimuli. Intratracheal instillation of recombinant mouse IL-36α induces CXCL1 and CXCL2 expression along with neutrophil influx in the lungs. IL-36α, IL-36β, and IL-36γ induce in vitro expression of the RN of multiple cytokines (IL-6, IL-12 p40, CXCL1, CCL1, IL-12 p35, IL-1β, IL-23 p19, GM-CSF, CXCL10, TNF-α, CCL3, VCAM-1, and ICAM-1) in mouse bone marrow-derived dendritic cells and CD4 T cells obtained from normal mice. IL-36α expression is elevated in chronic kidney disease and in rheumatoid arthritis synovium, and its decreased expression correlates with a poor prognosis in hepatocellular carcinoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human IL-36α (NP_055255) produced in HEK 293T cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565642 (BioLegend Cat. No. 676402)

Antigen Details

Structure
Full length IL-36α contains 167 amino acids with a predicted molecular weight of 17.7 kD. Proccessing at the N-terminal of IL-36α results in higher potency.
Distribution

Monocytes, T and B cells, spleen, bone marrow, tonsils, lymph nodes, skin, lung, fetal brain, stomach, and adipose tissue.

Function
IL-36α participates in inflammatory reactions, induces multiple cytokines in vivo and in vitro, and is involved in skin homeostasis and reduction of adipocyte differentiation. IL-17 and TNF induce IL-36α expression in keratinocytes, and IL-22 synergizes this induction. EGF also regulates the expression of IL-36α in the skin.
Interaction
Macrophages, adipocytes, lung fibroblasts, bone marrow dendritic cells, and T cells.
Ligand/Receptor
IL36R/IL-RAcP.
Cell Type
B cells, Dendritic cells, Monocytes, T cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Gresnigt MS and van de Veerdonk FL. 2013. Semin  Immunol. 6:458.
2. Towne JE and Sims JE. 2012. Curr. Opin. Pharmacol. 12:486.
3. Vigne S, et al. 2011. Blood 118:5813.
4. Towne JE, et al. 2011. J. Biol. Chem. 286:42594.
5. Chustz RT, et al. 2011. J. Respir. Cell Mol. Biol. 45:145.
6. Vos JB, et al. 2005. Physiol. Genomics 21:324.
7. Pan QZ, et al. 2013. Cancer Immunol. Immunother. 62:1675.

Gene ID
27179 View all products for this Gene ID
UniProt
View information about IL-36alpha on UniProt.org
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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