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- Clone
- 5A3A39 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon regulatory factor 9 (IRF-9), Interferon-stimulated gene factor 3 gamma (ISGF-3 gamma), ISGF3 p48 subunit
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total lysates (15 µg protein) from Jurkat (lane 1), Jurkat treated with IFNα1 (lane 2), THP-1 (lane 3) and Raw264.7 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1250 diluted (0.4 µg/mL) Purified anti-IRF9 Antibody, clone 5A3A39 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-IRF9 Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. (lower). Lane M: Molecular weight ladder. -
IFN-α treated Jurkat cells were stained with purified anti-IRF9 (5A3A39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody (Cat. No. 405310). Nuclei were stained with DAPI (blue). -
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with IFNα2 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF9 (Clone 5A3A39), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MX1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
IRF9 is a member of the IRF family of transcription factors and plays an important role in IFN signaling pathways and anti-viral immune responses. Type I and type III interferon stimulation induces phosphorylation and heterodimerization of STAT1 and STAT2. The activated STAT1:STAT2 heterodimer recruits IRF9 and forms a heterotrimeric complex termed IFN-stimulated gene factor 3 (ISGF3). The ISGF3 complex, in turn, translocates to the nucleus and transactivates IFN-inducible genes. The IRF9 protein in the ISGF3 complex is responsible for the recognition of IFN-stimulated response elements (ISRE). Mice lacking IRF9 fail to induce the expression of IFN regulated genes in response to type I interferon stimulation, indicating that IRF9 is crucial in mediating interferon signaling.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ChIP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 µg per mL. For ChIP applications, the suggested dilution is 1:50 to 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells
- Product Citations
-
- RRID
-
AB_2563575 (BioLegend Cat. No. 660702)
Antigen Details
- Structure
- 393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
- Distribution
-
Cytoplasm, phosphorylated form translocated to the nucleus.
- Function
- IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
- Interaction
- Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521.
2. Tsuno T, et al. 2009. J. Immunother. 32:803.
3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.
6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315. - Gene ID
- 10379 View all products for this Gene ID
- UniProt
- View information about IRF9 on UniProt.org
Other Formats
View All IRF9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-IRF9 | 5A3A39 | WB,ICC,ChIP |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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