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Purified anti-Keratin 10 Antibody

Pricing & Availability
Clone
Poly19054 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type I cytoskeletal 10, CK-10, keratin-10, cytokeratin 10
Isotype
Rabbit Polyclonal
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Product Citations
publications
Poly19054_PURE_Keratin10_Antibody_1_IHCP_062216
IHC staining of anti-Keratin 10 antibody (Poly19054) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
  • Poly19054_PURE_Keratin10_Antibody_1_IHCP_062216
    IHC staining of anti-Keratin 10 antibody (Poly19054) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by counterstaining with hematoxylin.
  • Poly19054_PURE_Keratin10_Antibody_2_WB_062216
    Western Blot of anti-Keratin 10 antibody (Poly19054). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature, followed by incubation with a fluorescent labeled goat anti-rabbit secondary antibody.
  • Poly19054_PURE_Keratin10_Antibody_3_WB_011918
    Total lysates (15 µg protein) from Raw264.7 (Mouse Low expression negative control) and Mouse skin (Positive control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Cytokeratin 10 antibody, clone Poly 19054. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Save
905403 25 µg ¥27,500
905404 100 µg ¥77,000
Description

Keratin 10 is a member of the type 1 (acidic) cytokeratin family. Keratins, in general, are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 10 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams JR, et al. 2011. Cancer Res. 71:2706.
  2. Lacroix M, et al. 2010. Proc. Natl. Acad. Sci. USA 107:21076-21081. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature 410:710.
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  5. Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
  6. Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Byrd KM, et al. 2020. Cell Stem Cell. 25(6):814-829.e6.. PubMed
  2. Koliakou E, et al. 2022. Int J Mol Sci. 23: . PubMed
  3. Yan Y, et al. 2023. Cancers (Basel). 15: . PubMed
  4. Chalmers FE, et al. 2022. Dev Biol. 485:9. PubMed
  5. Wong W, et al. 2022. Mol Metab. 65:101575. PubMed
  6. Al Moussawi K, et al. 2022. Cell Rep. 41:111503. PubMed
  7. Kantzer CG, et al. 2022. Development. 149:. PubMed
  8. Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed
  9. Wong W, et al. 2020. PLoS One. 15:e0241617. PubMed
  10. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  11. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  12. Villarreal-Ponce A, et al. 2020. Cell Rep. 33:108417. PubMed
  13. Nishiguchi MA et al. 2018. Cell reports. 24(13):3383-3392 . PubMed
  14. Koenig U, et al. 2019. Autophagy. :1. PubMed
  15. Lough KJ, et al. 2019. Elife. 8:e49249. PubMed
  16. Singh DR, et al. 2022. PLoS Pathog. 18:e1010868. PubMed
  17. Cai EY, et al. 2020. Cell Stem Cell. 27(2):270-283.e7. PubMed
RRID
AB_2616955 (BioLegend Cat. No. 905403)
AB_2616955 (BioLegend Cat. No. 905404)

Antigen Details

Structure
Keratin 10 is a 59 kD protein.
Distribution

Cytoskeleton, extracellular, and nucleus.

Function
Keratin 10 generally associates with keratin 1. Keratin 10 mutations are associated with epidermolytic hyperkeratosis.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3858 View all products for this Gene ID
UniProt
View information about Keratin 10 on UniProt.org
Go To Top Version: 3    Revision Date: 08/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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