- Clone
- W17021C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha), TCF1α, TCF10, TCF7L3
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
621051 | 25 µg | ¥26,400 | |
621052 | 100 µg | ¥64,900 |
Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream gene expression. LEF1 is implicated as a transcriptional regulator in the transformation of cancer cells in various diseases such as colonic adenocarcinoma. Due to its role in Wnt/β-catenin signaling pathway, LEF1 has been indicated as a facilitator of EMT and potential biomarker for various cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
LEF-1 is known to have 7 isoforms.
WB (Western blotting) or IHC-P (Immunohistochemistry FFPE) is not suggested for this product.
For WB, IP (immunoprecipitation) or ICC (immunocytochemistry) applications, please refer to clones 15H5A18 (Cat. No. 653101/653102) or W17155A (Cat. No. 616001/616002).
For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization. - RRID
-
AB_3083406 (BioLegend Cat. No. 621051)
AB_3083406 (BioLegend Cat. No. 621052)
Antigen Details
- Structure
- LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
- Distribution
-
Lymphoid tissue/Nuclear localization
- Function
- Canonical Wnt signaling
- Interaction
- Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
- Ligand/Receptor
- Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, TCRs
- Antigen References
-
- Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184-95.
- Waterman ML, et al. 1991. Genes Dev. 5:656-69.
- Hovanes K, et al. 2001. Nat Genet. 28:53-7..
- Bruhn L, et al. 1997. Genes Dev. 11:640-53.
- Nguyen A, et al. 2005. Int J Oncol. 27:949-56.
- Petropoulos K, et al. 2008. J Exp Med. 205:515-22.
- Gene ID
- 51176 View all products for this Gene ID
- UniProt
- View information about LEF1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All LEF1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-LEF1 | W17021C | ICFC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-LEF1
Jurkat cells (positive control) (filled histogram) and Daudi...
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