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Purified anti-Maf Antibody

Pricing & Availability
Clone
W18010A (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, T Lymphocyte C-Maf Long Form
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18010A_PURE_Maf_Antibody_1_031622
Whole cell extracts (15 µg total protein) from Jurkat, RPMI-8226, HEK293, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:500 dilution) of purified anti-Maf (clone W18010A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • W18010A_PURE_Maf_Antibody_1_031622
    Whole cell extracts (15 µg total protein) from Jurkat, RPMI-8226, HEK293, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:500 dilution) of purified anti-Maf (clone W18010A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • W18010A_PURE_Maf_Antibody_2_031622
    Jurkat cells (negative target) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with 0.03 µg/test of purified anti-Maf (clone W18010A) (filled histogram) or of purified rat IgG2a, κ isotype control (Cat. No. 400502) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W18010A_PURE_Maf_Antibody_3_031622
    RPMI-8226 cells (positive target) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with 0.03 µg/test of purified anti-Maf (clone W18010A) (filled histogram) or purified rat IgG2a, κ isotype control (Cat. No. 400502) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W18010A_PURE_Maf_Antibody_4_031622
    ICC staining of purified anti-Maf (clone W18010A) on RPMI-8226 cells. The cells were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton-X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective. Scale bar = 50 μm
Cat # Size Price Save
603451 25 µg ¥25,960
603452 100 µg ¥64,460
Description

Transcription factor Maf (Maf) is a transcription factor encoded by the MAF gene. In a complex as a homo- or hetero-dimer, Maf acts as a transcriptional activator and repressor with diverse functions. For example, Maf plays numerous roles in the immune system by regulating IL-4 expression and modulating T-cell apoptosis, and is also a critical regulator of glucagon production in pancreatic alpha cells. Additionally, Maf plays a role in the development of multiple tissues, including the eye, bone, and blood vessels. Mutations in Maf are known to cause congenital and juvenile cataracts. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant N-terminus fragment of human Maf
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is 0.03 - 0.05 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been tested and does not work for IHC-P. 

For ICC application, paraformaldehyde plus Triton-X and paraformaldehyde plus methanol fix/perm methods are recommended. The use of methanol only as fix/perm is not suitable for this antibody.

For ICFC application, Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended.

RRID
AB_2910482 (BioLegend Cat. No. 603451)
AB_2910482 (BioLegend Cat. No. 603452)

Antigen Details

Structure
Maf is a 373 amino-acid protein with a predicted molecular weight of 38.5 kD.
Distribution

Nucleus

Function
Transcription factor
Interaction
Maf has been shown to interact with CREBBP, EP300, MYB and SOX9.
Cell Type
Endothelial cells, Osteoblasts, T cells, Th2
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Transcription Factors
Antigen References
  1. Imbratta C, et al. 2020. Front Immunol. 11:206.
  2. Zhang C and Guo ZM. 2015. Diabetes Metab Res Rev. 31:773-8.
Gene ID
4094 View all products for this Gene ID
UniProt
View information about Maf on UniProt.org
Go To Top Version: 1    Revision Date: 03/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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