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- Clone
- 6F10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Leu2
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Marmoset peripheral blood lymphocytes were stained with purified CD8 (clone 6F10) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Verified Reactivity
- Common Marmoset
- Reported Reactivity
- Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Marmoset mononuclear cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The 6F10 antibody has been reported to cross-react with lymphocytes from squirrel monkeys but not from cynomolgus monkeys or humans.
Additional reported applications for the relevant formats include: iimmunohistochemical staining of frozen spleen sections1, Western blotting1, immunoprecipitation1. -
Application References
(PubMed link indicates BioLegend citation) -
- Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, IP, WB)
- RRID
-
AB_2562121 (BioLegend Cat. No. 250302)
Antigen Details
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 100387674 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
Related FAQs
Other Formats
View All CD8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-marmoset CD8 | 6F10 | FC,IHC-F,IP,WB |
| PE anti-marmoset CD8 | 6F10 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-marmoset CD8
Marmoset peripheral blood lymphocytes were stained with puri... -
PE anti-marmoset CD8
Marmoset peripheral blood lymphocytes were stained with CD8 ...
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