Purified anti-mouse CD100 SEMA4D Antibody anti-CD100

Clone: BMA-12 (See other available formats)
Regulatory Status: RUO
Other Names: Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
Isotype: Rat IgG2a, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with purified CD100/SEMA4D (clone BMA-12, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.

Cat #	Size	Price	Save
147601	25 µg	¥22,220
147602	100 µg	¥68,420

Description

CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD100-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC-F - Verified
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates², capture antibody function in a sandwich ELISA assay², and immunohistochemical staining of frozen brain sections⁴.
RRID: AB_2563413 (BioLegend Cat. No. 147601)
AB_2563413 (BioLegend Cat. No. 147602)

Antigen Details

Structure: Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Distribution: Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.
Function: Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
Ligand/Receptor: CD72, Plexin-B1.
Cell Type: B cells, Dendritic cells, Neutrophils, Tregs
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell. 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 20354 View all products for this Gene ID
UniProt: View information about CD100 on UniProt.org

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Description	Clone	Applications
Purified anti-mouse CD100 (SEMA4D)	BMA-12	FC,IHC-F,IP,ELISA
PE anti-mouse CD100 (SEMA4D)	BMA-12	FC
APC anti-mouse CD100 (SEMA4D)	BMA-12	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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