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- Clone
- W19330E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CSF-1R, M-CSR, c-fms
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with purified anti-mouse CD115 (clone W19330E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) followed by PE goat-anti-rat IgG.
CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD115 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone W19330E does not affect binding of clone W19330C on target cells.
Clone W19330E can block clone AFS98 binding on target cells.
Clone W19330E can block ligand binding to CSFR-1. - RRID
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AB_2910351 (BioLegend Cat. No. 165202)
Antigen Details
- Structure
- Ig superfamily, 145 kD
- Distribution
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Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Macrophage colony stimulating factor (M-CSF), IL-34
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Osteoclasts
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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- Guilbert LJ and Stanley ER. 1980. J Cell Bio. 85:153-9.
- Yeung Y-G, et al. 1978. Proc Natl Acad Sci. 84:1268-71.
- Sherr CJ, et al. 1985. Cell. 41:665-76.
- Gene ID
- 12978 View all products for this Gene ID
- UniProt
- View information about CD115 on UniProt.org
Other Formats
View All CD115 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD115 (CSF-1R) | W19330E | FC |
| PE anti-mouse CD115 (CSF-1R) | W19330E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
PE anti-mouse CD115 (CSF-1R)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w...
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