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- Clone
- A19116F (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Mouse Fas Ligand transfected cells were stained with purified anti-mouse CD178 (FasL) (clone A19116F) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG2a Alexa Fluor® 647.
CD178 is a 40 kD member of the TNF superfamily also known as Fas ligand or FasL. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant mouse FasL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone A19116F does not affect the binding of clone MFL3 on target cells.
- RRID
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AB_2936669 (BioLegend Cat. No. 134202)
Antigen Details
- Structure
- TNF superfamily, 40 kD
- Distribution
-
Activated T cells, spleen, testis, eye
- Function
- Induces apoptosis
- Ligand/Receptor
- Fas (CD95)
- Cell Type
- T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Nagata S. 1999. Annu Rev Genet. 33:29-55.
- Takahashi T, et al. 1994. Cell. 76:969-76.
- Hill LL, et al. 1999. Science. 285:898-900.
- Gene ID
- 14103 View all products for this Gene ID
- UniProt
- View information about CD178 on UniProt.org
Related FAQs
Other Formats
View All CD178 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD178 (FasL) | A19116F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD178 (FasL)
Mouse Fas Ligand transfected cells were stained with purifie...
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