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- Clone
- 3F10-B3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-8RB, GRO/MGSA, Cmkar2
- Isotype
- Mouse IgG2c, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G/Ly-6C APC and purified anti-mouse CD182 (CXCR2) (clone 3F10-B3) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a. -
Recombinant mouse CXCL2 (MIP-2) (carrier-free) (5 ng/mL) (Cat. No 582502) chemoattracts Baf3-mCXCR2 transfected cells (black circles) in a dose-dependent manner. Anti-mouse CXCR2 monoclonal antibody (clone 3F10-B3) inhibits the migration of Baf3-mCXCR2 transfected cells (purple circles), in a dose-dependent manner. The ED50 for this effect is 1 - 4 µg/mL.
CD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Pre-B cell transfected with mCXCR2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Block - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone 3F10-B3 blocks the chemotaxis towards CXCR2 ligands.
- RRID
-
AB_2924493 (BioLegend Cat. No. 163902)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions.
- Distribution
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Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK)
- Function
- Chemotaxis, neutrophil activation, and angiogenesis
- Ligand/Receptor
- CXCL1, CXCL2, CXCL3, and CXCL5
- Cell Type
- Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
- Spaan AN, et al. 2014. Nat Commun. 5:5438.
- Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064-9.
- Hsieh CY, et al. 2014. J Immunol. 193:3693-703.
- Gene ID
- 12765 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
Other Formats
View All CD182 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) | 3F10-B3 | FC,Block |
| Purified anti-mouse CD182 (CXCR2) | 3F10-B3 | FC,Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... 
Recombinant mouse CXCL2 (5 ng/mL) (Cat. No 582502) chemoattr... -
Purified anti-mouse CD182 (CXCR2)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... 
Recombinant mouse CXCL2 (MIP-2) (carrier-free) (5 ng/mL) (Ca...
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