Purified anti-mouse CD19 Maxpar Ready Antibody anti-CD19

Clone: 6D5 (See other available formats)
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

6D5_Purified_CD19_CyTOF_112113 — Mouse splenocytes stained with 169Tm-anti-TCRβ (H57-597) and 149Sm-anti-CD19 (6D5). Data provided by DVS Sciences.

Cat #	Size	Price	Save
115547	100 µg	¥20,680

Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence⁷.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2562806 (BioLegend Cat. No. 115547)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD19	6D5	FC
Biotin anti-mouse CD19	6D5	FC
FITC anti-mouse CD19	6D5	FC
PE anti-mouse CD19	6D5	FC
PE/Cyanine5 anti-mouse CD19	6D5	FC
Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19	6D5	FC
Alexa Fluor® 488 anti-mouse CD19	6D5	FC
Alexa Fluor® 647 anti-mouse CD19	6D5	FC,IHC-F
Pacific Blue™ anti-mouse CD19	6D5	FC
Alexa Fluor® 700 anti-mouse CD19	6D5	FC
APC/Cyanine7 anti-mouse CD19	6D5	FC
PerCP anti-mouse CD19	6D5	FC
PerCP/Cyanine5.5 anti-mouse CD19	6D5	FC
Alexa Fluor® 594 anti-mouse CD19	6D5	IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19	6D5	FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19	6D5	FC
Brilliant Violet 605™ anti-mouse CD19	6D5	FC
Brilliant Violet 650™ anti-mouse CD19	6D5	FC
Brilliant Violet 785™ anti-mouse CD19	6D5	FC
Brilliant Violet 510™ anti-mouse CD19	6D5	FC
Purified anti-mouse CD19 (Maxpar® Ready)	6D5	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19	6D5	FC
Brilliant Violet 711™ anti-mouse CD19	6D5	FC
APC/Fire™ 750 anti-mouse CD19	6D5	FC
TotalSeq™-A0093 anti-mouse CD19	6D5	PG
Brilliant Violet 750™ anti-mouse CD19	6D5	FC
TotalSeq™-B0093 anti-mouse CD19	6D5	PG
Spark Blue™ 550 anti-mouse CD19	6D5	FC
Spark NIR™ 685 anti-mouse CD19	6D5	FC
TotalSeq™-C0093 anti-mouse CD19	6D5	PG
Ultra-LEAF™ Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody	6D5	FC
Spark YG™ 581 anti-mouse CD19	6D5	FC
APC/Fire™ 810 anti-mouse CD19	6D5	FC
Spark YG™ 570 anti-mouse CD19	6D5	IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody	6D5	FC
Spark Blue™ 515 anti-mouse CD19	6D5	FC
Spark UV™ 387 anti-mouse CD19	6D5	FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™)	6D5	FC

Certificate of Analysis

Customers Also Purchased

Purified anti-mouse Ly-6C (Maxpar® Ready)

Clone: HK1.4

Purified anti-mouse TCR β chain

Clone: H57-597

Purified anti-mouse CD88 (C5aR)

Clone: 20/70

APC/Cyanine7 anti-mouse Ly-6C

Clone: HK1.4

Go To Top Version: 2 Revision Date: 03/05/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.