- Clone
- 7D4/CD25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, IL2RA, IL-2Ra, Ly-43, p55, Tac
- Isotype
- Rat IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![7D4slashCD25_PURE_CD25_Antibody_1_091417 7D4slashCD25_PURE_CD25_Antibody_1_091417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/7D4slashCD25_PURE_CD25_Antibody_1_091417.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stimulated with Con A + IL-2 for 3 days and then stained with CD3 Alexa Fluor® 647 and purified CD25 (clone 7D4/CD25, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM-FITC. -
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C57BL/6 mouse splenocytes stained with CD3 Alexa Fluor® 647 and purified CD25 (clone 7D4/CD25, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM-FITC. -
CD25, also known as IL-2 low affinity receptor is a type I, 55 kDa, transmembrane glycoprotein. CD25 It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- HT-2 cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
Block, Depletion, IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.
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Application References
(PubMed link indicates BioLegend citation) -
- Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP)
- Couper KN. 2007. J. Immunol. 178:4136. (Depletion)
- Product Citations
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- RRID
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AB_2716173 (BioLegend Cat. No. 154202)
Antigen Details
- Structure
- Type I, 55 kD, transmembrane glycoprotein
- Distribution
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Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Huss DJ, et al. 2015. J. Immunol. 194:84.
2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056.
3. Codarri L, et al. 2007. J. Exp. Med. 204:1533.
4. Read S, et al. 2000. J. Exp. Med. 192:295.
5. Taniguchi T, et al. 1993. Cell 73:5. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
Other Formats
View All CD25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD25 | 7D4/CD25 | FC,Block,Depletion,IP |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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