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Purified anti-mouse CD25 Recombinant Antibody

Pricing & Availability
Clone
QA19A49 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, and Tac
Isotype
Rat IgG2a, κ
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Product Citations
publications
QA19A49_PURE_CD25_Antibody_031921
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 FITC and purified anti-mouse CD25 recombinant (clone QA19A49) (left) or rat IgG2a, ᴋ isotype control (right), followed by anti-rat IgG PE.
  • QA19A49_PURE_CD25_Antibody_031921
    C57BL/6 mouse splenocytes were stained with anti-mouse CD4 FITC and purified anti-mouse CD25 recombinant (clone QA19A49) (left) or rat IgG2a, ᴋ isotype control (right), followed by anti-rat IgG PE.
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Cat # Size Price Save
162102 100 µg ¥18,260
Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα. CD25 is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone binds to mouse CD25, also known as the low affinity IL-2Rα.

RRID
AB_2890727 (BioLegend Cat. No. 162102)

Antigen Details

Structure
CD25 is a 55 kD glycoprotein.
Distribution

B cells, T cells, Thymocytes, Tregs

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 1    Revision Date: 03/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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