- Clone
- RMP1-30 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Programmed Death-1, CD279, PDCD1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMP1-30_Purified_CD279_Cytof_060314.jpg RMP1-30_Purified_CD279_Cytof_060314.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMP1-30_Purified_CD279_Cytof_060314.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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BALB/c mouse splenocytes were incubated for 3 days in media alone (bottom) or with Concanavalin A (top) and then stained with 159Tb-anti-CD279 (RMP1-30). Data provided by DVS Sciences.
CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-1 transfected BHK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2563735 (BioLegend Cat. No. 109113)
Antigen Details
- Structure
- Ig superfamily, 50-55 kD
- Distribution
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Subset of double negative thymocytes, activated T and B cells
- Function
- Lymphocyte clonal selection, peripheral tolerance
- Ligand/Receptor
- PD-L1 (B7-H1), PD-L2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887. - Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD279 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD279 (PD-1) | RMP1-30 | FC |
Purified anti-mouse CD279 (PD-1) | RMP1-30 | FC,IHC-F |
Biotin anti-mouse CD279 (PD-1) | RMP1-30 | FC |
LEAF™ Purified anti-mouse CD279 (PD-1) | RMP1-30 | FC, FA |
PE/Cyanine7 anti-mouse CD279 (PD-1) | RMP1-30 | FC |
APC anti-mouse CD279 (PD-1) | RMP1-30 | FC |
Purified anti-mouse CD279 (PD-1) (Maxpar® Ready) | RMP1-30 | FC,CyTOF® |
PE/Dazzle™ 594 anti-mouse CD279 (PD-1) | RMP1-30 | FC |
Alexa Fluor® 647 anti-mouse CD279 (PD-1) | RMP1-30 | FC |
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1) | RMP1-30 | FC |
Brilliant Violet 421™ anti-mouse CD279 (PD-1) | RMP1-30 | FC |
TotalSeq™-A0004 anti-mouse CD279 (PD-1) | RMP1-30 | PG |
TotalSeq™-B0004 anti-mouse CD279 (PD-1) | RMP1-30 | PG |
TotalSeq™-C0004 anti-mouse CD279 (PD-1) | RMP1-30 | PG |
KIRAVIA Blue 520™ anti-mouse CD279 (PD-1) | RMP1-30 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD279 (PD-1)
Con A (3-day) activated C57BL/6 mouse splenocytes stained wi... -
Purified anti-mouse CD279 (PD-1)
Con A (3-day) activated C57BL/6 mouse splenocytes stained wi... Fresh, frozen mouse spleen was stained with purified CD279 c... -
Biotin anti-mouse CD279 (PD-1)
Con A (3-day) activated C57BL/6 mouse splenocytes stained wi... -
LEAF™ Purified anti-mouse CD279 (PD-1)
-
PE/Cyanine7 anti-mouse CD279 (PD-1)
Con A-stimulated (day-3) Balb/c mouse splenocytes stained wi... -
APC anti-mouse CD279 (PD-1)
Con A-stimulated C57BL/6 splenocytes (Day 3) stained with RM... -
Purified anti-mouse CD279 (PD-1) (Maxpar® Ready)
BALB/c mouse splenocytes were incubated for 3 days in media ... -
PE/Dazzle™ 594 anti-mouse CD279 (PD-1)
Con A (three-days) activated C57BL/6 splenocytes were staine... -
Alexa Fluor® 647 anti-mouse CD279 (PD-1)
Con A (three-days) activated C57BL/6 splenocytes were staine... -
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
Brilliant Violet 421™ anti-mouse CD279 (PD-1)
Con A (three-days) activated C57BL/6 splenocytes were staine... -
TotalSeq™-A0004 anti-mouse CD279 (PD-1)
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TotalSeq™-B0004 anti-mouse CD279 (PD-1)
-
TotalSeq™-C0004 anti-mouse CD279 (PD-1)
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KIRAVIA Blue 520™ anti-mouse CD279 (PD-1)
Con A (three-days) activated C57BL/6 splenocytes were staine...
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