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Purified anti-mouse DLK1 Antibody

Pricing & Availability
Clone
W21075D (See other available formats)
Regulatory Status
RUO
Other Names
Pref, protein delta homolog 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W21075D_PURE_DLK1_FC_082924
Mouse NIH/3T3 fibroblast cells were stained with purified anti-mouse DLK1 (clone W21075D) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • A. W21075D_PURE_DLK1_FC_082924
    Mouse NIH/3T3 fibroblast cells were stained with purified anti-mouse DLK1 (clone W21075D) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • B. W21075D_PURE_DLK1_ICC_082924.
    Mouse NIH/3T3 fibroblast cells were fixed with 4% PFA Fixation Buffer, washed with PBS and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with purified anti-mouse DLK1 (clone W21075D, panel A) or 2.5 µg/mL purified rat IgG2b, κ isotype control (panel B) followed by 2.5 µg/mL of Alexa Fluor 555® anti-mouse IgG (Cat. No. 405324) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40x objective. Scale bar: 20 µm
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Cat # Size Price Save
122302 100 µg ¥94,600
Description

The Delta-Notch pathway controls developmental processes including cell fate determination, terminal differentiation, and proliferation. In mammals, four Notch receptors (NOTCH1–4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described. Delta-like 1 homolog (DLK1) is a noncanonical Notch ligand, and acts as an inhibitor of Notch signaling. The inhibitory effect of DLK1 and DLK2 on Notch signaling may be mediated by competition with canonical ligands for the receptor binding site. Widely expressed during embryonic development, expression becomes restricted in the adult to neuroendocrine tissues like the pituitary gland, adrenal glands, pancreas, monoaminergic neurons in the central nervous system, testes, prostate, and ovaries.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mDLK1-RBL1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Single-pass type I membrane protein
Distribution

Cytoplasm, plasma membrane

Function
Neuroendocrine differentiation, inhibits adipocyte differentiation
Interaction
Notch receptors
Ligand/Receptor
Notch receptors
Cell Type
Fibroblasts, Neurons
Biology Area
Neuroscience, Signal Transduction
Antigen References
  1. Smas C.M, et al. 1993. Cell. 73:725-34.
  2. Laborda J, et al. 1993. J Biol Chem. 268:3817-20.
  3. Montalban-Loro R, et al. 2021. Proc Natl Acad Sci USA. 118 (11).
  4. Zhang L, et al. 2021. Stem Cells. 39:306-17.
  5. Ichinose M, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 320:G506-20.
Gene ID
13386 View all products for this Gene ID
UniProt
View information about DLK1 on UniProt.org
Go To Top Version: 1    Revision Date: 08/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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