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- Clone
- 15-5-5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I
- Isotype
- Mouse (C3H.SW) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a FITC.
The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
-
The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.
This clone does not cross react with other haplotypes (e.g., a, b, p, q, r, s). -
Application References
(PubMed link indicates BioLegend citation) -
- Ozato K, et al. 1980. J. Immunol. 124:533.
- Yamamoto M, et al. 1998. J. Immunol. 160:2188.
- Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed
- Product Citations
-
- RRID
-
AB_313475 (BioLegend Cat. No. 110302)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Dk haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. - Gene ID
- 14964 View all products for this Gene ID
- UniProt
- View information about H-2Dk on UniProt.org
Other Formats
View All H-2Dk Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse H-2Dk | 15-5-5 | FC |
| Purified anti-mouse H-2Dk | 15-5-5 | FC,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse H-2Dk
C3H/He mouse splenocytes were stained with H-2Dk ... -
Purified anti-mouse H-2Dk
C3H/He mouse splenocytes were stained with purified H-2D
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