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- Clone
- MK4.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Raw 264.7 cells were stimulated with human TRANCE for 4 days (filled histogram) or were cultured without stimulus (open histogram) and were then stained with purified anti-mouse/human Siglec-15 (clone MK4.5), followed by PE anti-mouse IgG1.
Siglec-15, also known as Siglec-I and CD33L3, is a member of the Sialic acid-binding Ig-like lectin family and is a type I single pass transmembrane protein. Siglec-15 is expressed by a subset of myeloid cells, osteoclasts, and can be upregulated by certain cancers. Siglec-15 plays an important role in osteoclastogenesis and bone remodeling during normal development and in bone absorption in arthritis. Furthermore, Siglec-15 suppresses antigen-specific T cell responses and is therefore considered to be an immune suppressor and potential target for normalization cancer immunotherapy.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Siglec-15-huIgG Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
ICC, ICFC, Direct ELISA, ELISA Capture - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Korn M, et al. 2020. J Immunol. 205:2595-2605.
- RRID
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AB_2922502 (BioLegend Cat. No. 164602)
Antigen Details
- Structure
- Type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail
- Distribution
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Myeloid lineage, osteoclasts, and upregulated by certain types of human cancers
- Function
- Regulates osteoclast development and bone resorption
- Interaction
- DAP12
- Ligand/Receptor
- Neu5Ac alpha2-6GalNAc alpha
- Cell Type
- Macrophages, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- Siglec Molecules
- Antigen References
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- Wang J, et al. 2019. Nat Med. 25:656-666.
- Angata T. 2020. J Biomed Sci. 27:10.
- Takamiya R, et al. 2013. Glycobiology. 23:178-87.
- Gene ID
- 620235 View all products for this Gene ID 284266 View all products for this Gene ID
- UniProt
- View information about Siglec-15 on UniProt.org
Related FAQs
Other Formats
View All Siglec-15 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse/human Siglec-15 | MK4.5 | FC,ICFC,ICC,ELISA Capture,Direct ELISA |
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Purified anti-mouse/human Siglec-15
Raw 264.7 cells were stimulated with human TRANCE for 4 days...
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