United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Clone
- MK4.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Raw 264.7 cells were stimulated with human TRANCE for 4 days (filled histogram) or were cultured without stimulus (open histogram) and were then stained with purified anti-mouse/human Siglec-15 (clone MK4.5), followed by PE anti-mouse IgG1.
Siglec-15, also known as Siglec-I and CD33L3, is a member of the Sialic acid-binding Ig-like lectin family and is a type I single pass transmembrane protein. Siglec-15 is expressed by a subset of myeloid cells, osteoclasts, and can be upregulated by certain cancers. Siglec-15 plays an important role in osteoclastogenesis and bone remodeling during normal development and in bone absorption in arthritis. Furthermore, Siglec-15 suppresses antigen-specific T cell responses and is therefore considered to be an immune suppressor and potential target for normalization cancer immunotherapy.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Siglec-15-huIgG Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, ICFC, Direct ELISA, ELISA Capture - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
- Korn M, et al. 2020. J Immunol. 205:2595-2605.
- RRID
-
AB_2922502 (BioLegend Cat. No. 164602)
Antigen Details
- Structure
- Type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail
- Distribution
-
Myeloid lineage, osteoclasts, and upregulated by certain types of human cancers
- Function
- Regulates osteoclast development and bone resorption
- Interaction
- DAP12
- Ligand/Receptor
- Neu5Ac alpha2-6GalNAc alpha
- Cell Type
- Macrophages, Osteoclasts
- Biology Area
- Immunology
- Molecular Family
- Siglec Molecules
- Antigen References
-
- Wang J, et al. 2019. Nat Med. 25:656-666.
- Angata T. 2020. J Biomed Sci. 27:10.
- Takamiya R, et al. 2013. Glycobiology. 23:178-87.
- Gene ID
- 620235 View all products for this Gene ID 284266 View all products for this Gene ID
- UniProt
- View information about Siglec-15 on UniProt.org
Other Formats
View All Siglec-15 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse/human Siglec-15 | MK4.5 | FC,ICFC,ICC,ELISA Capture,Direct ELISA |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse/human Siglec-15
Raw 264.7 cells were stimulated with human TRANCE for 4 days...
Follow Us