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Purified anti-mouse IL-1α Antibody

Pricing & Availability
Clone
ALF-161 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
Mouse_IL-1alpha_020305
  • Mouse_IL-1alpha_020305
  • zALF-161_IF_072412
    Immortalized murine bone marrow-derived macrophages stimulated with LPS (100 ng/ml) for 24 h, fixed with 2% paraformaldehyde, permeabilized with 0.1% Triton X100, stained with IL-1α (clone ALF-161), followed by DyLight® 594 goat anti-hamster Ig (red). Cells also express GFP-LC3 (green). Image was visualized on an Olympus FV1000 confocal at 63x. Images provided by James Harris and Celia Peral de Castro, Trinity College.
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Cat # Size Price Save
503202 500 µg ¥29,920
Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The ALF-161 antibody reacts with precursor, secreted and membrane-associated forms of mouse IL-1α. The ALF-161 antibody can neutralize the bioactivity of natural or recombinant IL-1α.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli - expressed, recombinant mouse IL-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IF - Verified
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5034 antibody (Cat. No. 503401) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
ELISA Capture: The purified ALF-161 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
Neutralization: Clone ALF-161 has been described to have IL-1a neutralizing properties based on the in vivo mice model3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended (Cat. No. 503207-503212).

Application References

(PubMed link indicates BioLegend citation)
  1. Fuhlbrigge R, et al. 1988. J. Immunol. 141:2643.
  2. Rogers HW, et al. 1992. P. Natl. Acad.Sci. USA 89:1011.
  3. Yazdi AS, et al. 2010. P. Natl. Acad. Sci. USA 107:19449. (Neut)
RRID
AB_315280 (BioLegend Cat. No. 503202)

Antigen Details

Structure
Cytokine; 17.4 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets
B cells, T cells, monocytes, fibroblasts
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
16175 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-1α Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-1α ALF-161 ICFC
Purified anti-mouse IL-1α ALF-161 ELISA Capture,IP,WB
Ultra-LEAF™ Purified anti-mouse IL-1α ALF-161 ELISA Capture,ELISPOT Capture,ICFC,IP,Neut,WB
Go To Top Version: 2    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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