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Purified anti-mouse IL-21 Antibody

Pricing & Availability
Clone
S20017B (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-21
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
S20017B_PURE_IL-21_Antibody_040523
PMA+ ionomycin-stimulated (4 hours, in presence of brefeldin A) Th17-polarized C57BL/6 mouse CD4 T cells were stained with anti-mouse CD3ɛ APC. After fixation and permeabilization, cells were stained intracellularly with purified anti-mouse IL-21 (clone S20017B) (left) or purified rat IgG1, κ isotype control (right), followed by anti-rat IgG1 PE.
  • S20017B_PURE_IL-21_Antibody_040523
    PMA+ ionomycin-stimulated (4 hours, in presence of brefeldin A) Th17-polarized C57BL/6 mouse CD4 T cells were stained with anti-mouse CD3ɛ APC. After fixation and permeabilization, cells were stained intracellularly with purified anti-mouse IL-21 (clone S20017B) (left) or purified rat IgG1, κ isotype control (right), followed by anti-rat IgG1 PE.
Cat # Size Price Save
122202 100 µg ¥76,340
Description

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT cells and CD4+ T cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941414 (BioLegend Cat. No. 122202)

Antigen Details

Structure
Interleukin
Distribution

CD4 activated T cells, Th17 cells, Th2 cells

Function
Immunomodulation
Ligand/Receptor
IL-21R
Cell Type
NKT cells, T cells, Tfh, Th17, Th2
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Parrish-Novak J, et al. 2000. Nature. 408:57-63.
  2. Mehta DS, et el. 2004. Immunol Rev. 202:84-95.
Gene ID
60505 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/05/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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