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Purified anti-mouse IL-22 Antibody

Pricing & Availability
Clone
BL35175 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-22, IL-TIF, IL-10-related T-cell-derived-inducible factor
Isotype
Rat IgG2a, κ
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Product Citations
publications
BL35175_PURE_IL22_Antibody_WB_050416
Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
  • BL35175_PURE_IL22_Antibody_WB_050416
    Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
Cat # Size Price Save
517501 25 µg ¥22,080
517502 100 µg ¥47,170
Description

IL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD.  Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mature recombinant protein mouse Interleukin-22 (Leu34-Val179)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml dilution buffer for each mini-gel.

Application Notes

Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.

Product Citations
  1. Ogita T, et al. 2021. Front Nutr. 8:701466. PubMed
  2. Ke Y, et al. 2011. J Immunol. 187:2130. PubMed
  3. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
RRID
AB_10695475 (BioLegend Cat. No. 517501)
AB_10695475 (BioLegend Cat. No. 517502)

Antigen Details

Structure
179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.
Distribution

Secreted, Th17

Function
Cytokine that contributes to the inflammatory response in vivo.
Ligand/Receptor
Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.
Cell Type
Th17
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure 16:1333.
4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293.
5. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
6. Chang C, et al. 2006. Cell Research 16:902.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org
Go To Top Version: 3    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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