Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody, Ly-6A/E, QA17A36

Clone: QA17A36 (See other available formats)
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG1, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with purified Ly-6A/E (clone QA17A36, filled histogram) or purified rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG1 PE Antibody.

Cat #	Size	Price	Save
163802	100 µg	¥22,220

Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910344 (BioLegend Cat. No. 163802)

Antigen Details

Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Antigen References: Upadhyay G. 2019. Front Immunol. 10:819.

Morrison SJ, et al. 1994. Immunity. 1:661.

Spangrude GJ, et al. 1988. J Immunol. 141:3697.
Gene ID: 110454 View all products for this Gene ID
UniProt: View information about Ly-6A/E on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Ly-6A/E Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC
PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC
APC anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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