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- Clone
- MK34 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6K, Ly-6K, mLy-6K
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Ly6K transfected-HEK-293 cells were stained with clone MK34 (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE. -
Mouse testicular tissue was formalin-fixed and paraffin-embedded, prior to sectioning. After deparaffinization and antigen retrieval with sodium citrate pH 6, the sample was stained overnight with anti-Ly6k (clone MK34) at 10 µg/ml followed by detection using BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC).
Lymphocyte antigen 6K (Ly6K) is a 17 kD GPI-anchored membrane protein expressed by testicular germ cells. Ly6K associates with TEX101 and may have a role in spermatogenesis. It is also expressed in some cancer cell types; a recent study also demonstrated its expression in plasma cells. A soluble 12 kD form of Ly6K has been reported.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse testicular homogenate.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1, western blotting1, and immunoprecipitation1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Maruyama M, et al. 2010. Biochem. Biophys. Res. Commun. 402:75. (IHC-F, IP, WB)
- RRID
-
AB_2616648 (BioLegend Cat. No. 151302)
Antigen Details
- Structure
- GPI-anchored membrane protein of 17 kD, soluble protein of 12 kD.
- Distribution
- Testicular germ cells, some cancer cell types, and plasma cells.
- Function
- Possible role in spermatogenesis.
- Interaction
- TEX101.
- Cell Type
- Plasma cells, B cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology
- Antigen References
-
1. Fujihara Y, et al. 2014. Biol. Reprod. 90:60.
2. von Allmen CE, et al. 2008. Mol. Immunol. 45:2727.
3. Yoshitake H, et al. 2008. Biochem. Biophys. Res. Commun. 372:277.
4. de Nooij-van Dalen AG, et al. 2003. Int. J. Cancer 103:768. - Gene ID
- 76486 View all products for this Gene ID
- UniProt
- View information about Ly6K on UniProt.org
Other Formats
View All Ly6K Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Ly6K | MK34 | FC,IHC-P,IHC-F,IP,WB |
| PE anti-mouse Ly6K | MK34 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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