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- Clone
- S20018D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MSP receptor, CD136, RON, STK
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 resident mouse peritoneal macrophages were stained with purified anti-mouse CD136 (Mst1r) (clone S20018D) (left) or purified rat IgG1, κ isotype control (right), followed by anti-rat IgG PE. Cells were costained with anti-mouse F4/80 APC.
Macrophage-stimulating 1 receptor (MST1R/RON) is a receptor protein kinase that transduce signals from the extracellular matrix into the cytoplasm by binding to the macrophage-stimulating protein (MSP). MST1R regulates various physiological processes including cell adhesion, motility, growth, and survival. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, it interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. RON plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than the MST1 ligand.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- MST1R transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2936598 (BioLegend Cat. No. 112402)
Antigen Details
- Structure
- The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35 kD (alpha) and 150 kD (beta) subunits
- Distribution
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Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
- Function
- Cell adhesion, motility, differentiation and host defense
- Interaction
- Macrophages, dendritic cells, granulocytes, hematopoietic stem and progenitors
- Ligand/Receptor
- MST1 ligand
- Molecular Family
- CD Molecules
- Antigen References
-
- Nikolaidis NM, et al. 2011. Innate Immun. 17:499–507.
- Bezerra JA, et al. 1993. Hepatology. 18:394–9.
- Gene ID
- 19882 View all products for this Gene ID
- UniProt
- View information about CD136 on UniProt.org
Related FAQs
Other Formats
View All CD136 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD136 (Mst1r) | S20018D | FC |
| PE anti-mouse CD136 (Mst1r) | S20018D | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD136 (Mst1r)
C57BL/6 resident mouse peritoneal macrophages were stained w... -
PE anti-mouse CD136 (Mst1r)
C57BL/6 resident mouse peritoneal macrophages were stained w...
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