Purified anti-mouse/rat/human MCP-1 Antibody anti-CCL2

Clone: 2H5 (See other available formats)
Regulatory Status: RUO
Other Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype: Hamster IgG
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Product Citations: publications

Cat #	Size	Price	Save
505901	50 µg	¥20,680
505902	500 µg	¥29,920

Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Rat, Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Hamster
Immunogen: CHO-expressed, recombinant mouse MCP-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
WB, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5-10 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.
Application Notes: ELISA or ELISPOT Capture¹: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry²: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization^1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting³, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections⁵, and acetone-fixed frozen tissue sections⁷.
RRID: AB_315408 (BioLegend Cat. No. 505901)
AB_315408 (BioLegend Cat. No. 505902)

Antigen Details

Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID: 20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
UniProt: View information about CCL2 on UniProt.org

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Other Formats

View All CCL2 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse/rat/human MCP-1	2H5	ICFC
Purified anti-mouse/rat/human MCP-1	2H5	ELISA Capture,IHC,WB
Biotin anti-mouse/rat/human MCP-1	2H5	ELISA Detection,ELISPOT Detection
APC anti-mouse/rat/human MCP-1	2H5	ICFC
Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1	2H5	ELISA Capture,ELISPOT Capture,IHC,Neut,WB

Certificate of Analysis

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Go To Top Version: 7 Revision Date: 08/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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