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Purified anti-MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
A15105J (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15105J_PURE_MRP-14_Antibody_WB_010218
Recombinant human S100A8 protein (Cat. No. 719902, lane 1), S100A9 protein( lane 2) and total lysates (15 µg protein) from HeLa (negative control, lane 3) , PBMC (lane 4) were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/mL) diluted purified anti-MRP-14 (S100A9) (clone A10105J). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • A15105J_PURE_MRP-14_Antibody_WB_010218
    Recombinant human S100A8 protein (Cat. No. 719902, lane 1), S100A9 protein( lane 2) and total lysates (15 µg protein) from HeLa (negative control, lane 3) , PBMC (lane 4) were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/mL) diluted purified anti-MRP-14 (S100A9) (clone A10105J). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Save
600301 25 µg ¥22,220
600302 100 µg ¥55,660
Description

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human S100A8/S100A9 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per mL (1:1000-1:10000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize S100A8. The 114 amino acid recombinant S100A9 protein has a predicted Mw of ~13 kD for monomer and ~26 kD for dimer.

Product Citations
  1. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID
AB_2721746 (BioLegend Cat. No. 600301)
AB_2721746 (BioLegend Cat. No. 600302)

Antigen Details

Structure
Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8. S100A9 has 114 amino acids with the predicted Mw of ~13 kDa.
Distribution

MDSCs, neutrophils, monocyte, and ileal tissue eosinophils

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction
Calcium, S100A8
Ligand/Receptor
RAGE, TLR4
Cell Type
Neutrophils, Monocytes, Eosinophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References

1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al. 2006. J. Mol. Biol. 359:961-72.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176.

Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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