Purified anti-Nestin Antibody anti-Nestin

Clone: Rat-401 (See other available formats)
Regulatory Status: RUO
Other Names: Nestin, NES
Isotype: Mouse IgG1, κ
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IHC staining of purified anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded embryonic mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

Cat #	Size	Price	Save
655102	100 µg	¥58,300

Description

Nestin was originally identified as a neuronal stem cell marker during central nervous system (CNS) development. It belongs to an intermediate filament protein family, and it is the most widely used marker for identifying neuronal stem cells. Nestin is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. However, the exact function of nestin is not very clear. Nestin is involved in structural organization of the cells through assembling into heterodimers with vimentin or α-internexin in a phosphorylation-dependent process to form intermediate filaments. The expression of nestin is downregulated and replaced by expression of the neurofilament proteins upon neural differentiation/maturation.

Expression of nestin has also been reported in non-neuronal immature or progenitor cells such as pancreatic islet progenitor cells, angiogenic endothelial cells, glioma cells, and bone marrow mesenchymal stem cells. Recent studies also showed that nestin expression can be detected in repair processes in the CNS and in various tumor cells. Thus, it has been used as a cancer stem cell marker. The usage of nestin as a novel prognostic indicator and possible target for tumor anigogenesis has been also proposed.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Homogenized Sprague-Dawley rat spinal cord.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The reactivity of Alexa Fluor® 647 anti-Nestin antibody was verified in FFPE embryonic mouse tissue.
RRID: AB_2562023 (BioLegend Cat. No. 655102)

Antigen Details

Structure: Mouse nestin is 1864 amino acids with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
Distribution: Cytosol
Function: Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References: 1. Lendahl U, et al. 1990. Cell 60:585.
2. Gilyarov AV, et al. 2008. Neurosci. Behav. Physiol. 38:165.
3. Méndez-Ferrer S, et al. 2010. Nature 466:829.
4. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
Gene ID: 18008 View all products for this Gene ID 25491 View all products for this Gene ID
UniProt: View information about Nestin on UniProt.org

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Other Formats

View All Nestin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nestin	Rat-401	IHC-P,IP
Alexa Fluor® 594 anti-Nestin	Rat-401	IHC-P
Alexa Fluor® 647 anti-Nestin	Rat-401	IHC-P

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 04/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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