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Purified anti-NTAL (LAT2) Antibody

Pricing & Availability
Clone
W15102A (See other available formats)
Regulatory Status
RUO
Other Names
NTAL, LAT2, WBS15, LAB
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_W15102A_PURE_NTAL_Antibody_071817
Total cell lysates from Raw264.7 (negative control, Lane 1), HEK293T (negative control, Lane 2), Daudi (Lane 3) , and THP-1 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL (1:500) purified anti-NTAL (LAT2) clone 15102A (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (cat #405301) for clone W15102A, and HRP donkey anti-rabbit IgG (cat #406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat no. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder.
  • 1_W15102A_PURE_NTAL_Antibody_071817
    Total cell lysates from Raw264.7 (negative control, Lane 1), HEK293T (negative control, Lane 2), Daudi (Lane 3) , and THP-1 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL (1:500) purified anti-NTAL (LAT2) clone 15102A (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (cat #405301) for clone W15102A, and HRP donkey anti-rabbit IgG (cat #406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat no. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder.
  • 2_W15102A_PURE_NTAL_Antibody_2_FC-Validated_120817
    Human peripheral blood mononuclear cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with purified anti-NTAL (LAT2) antibody (clone W15102A (filled histogram) or purified mouse IgG1, κ isotype control (clone MOPC-21) (dashed histogram) followed by PE goat anti-mouse IgG antibody (clone Poly4053). Lyphocyte population gating (top), monocyte population gating (bottom).
  • 3_W15102A_PURE_NTAL_Antibody_3_FC-Validated_120817
Compare all formats
Cat # Size Price Save
699001 25 µg ¥22,220
699002 100 µg ¥55,660
Description

Non-T cell activation linker (NTAL) functions as transmembrane adaptor scaffold that links engagement of antigen receptor binding to downstream effector molecules, playing an essential role in maturation, activation, and differentiation in various cell types. It is important for BCR (B-cell antigen receptor) and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-signaling in B-cells and myeloid cells, respectively, and plays a critical role in FCER1 (high affinity immunoglobulin epsilon receptor) signaling in mast cells.  NTAL is encoded by the LAT2 gene, which is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NTAL recombinant protein (27-243 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-5000). For flow cytometric staining, the suggested use of this reagent is ≤0.00375 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not cross-react with mouse NTAL (in-house tested). Daudi cells were selected for antibody testing due to the cell line’s low expression level of the mRNA encoding NTAL (source: Human Protein Atlas).

RRID
AB_2715811 (BioLegend Cat. No. 699001)
AB_2715811 (BioLegend Cat. No. 699002)

Antigen Details

Structure
243 amino acids with a predicted molecular weight of 26.5 kD, palmitoylation moiety permits localization to lipid rafts or tetraspanin-enriched microdomains.
Distribution

Plasma membrane

Function
NTAL is extensively phosphorylated on multiple tyrosine residues following activation, enabling the protein to serve as a scaffold for multiple signaling complexes in various cell contexts. May also be post-translationally regulated by Caspase-3-mediated proteolytic cleavage in human B cells and monocytes.
Interaction
Phospholipase C gamma 1 and 2. When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1, and CBL.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Arbulo-Echevarria MM, et al. 2016. J. Leukoc Biol. 100:351.
2. Stepanek O, et al. 2014. Cell Signal. 5:895.
3. Orr Sj, et al. 2013. Plos Pathog. 9: e1003357.
4. Halova I, et al. 2013. J. Biol. Chem. 288:9801.
5. Duque-Alfonso J, et al. 2011. Oncogene. 30:3062 

Gene ID
7462 View all products for this Gene ID
UniProt
View information about NTAL on UniProt.org
Go To Top Version: 0    Revision Date: 07/19/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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