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Purified anti-PAX9 Antibody

Pricing & Availability
Clone
14D10A24 (See other available formats)
Regulatory Status
RUO
Other Names
Paired box protein Pax-9
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14D10A24_PAX9_Purified_Antibody_1_WB_070918
Total cell lysates (15 µg protein) from HeLa (lane 1), PC3 (lane 2), Jurkat (Lane 3) and NIH3T3 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-Pax9 Antibody, clone 14D10A24 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-Pax9 Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower) as a loading control. Lane M: Molecular weight ladder.
  • 14D10A24_PAX9_Purified_Antibody_1_WB_070918
    Total cell lysates (15 µg protein) from HeLa (lane 1), PC3 (lane 2), Jurkat (Lane 3) and NIH3T3 cells (lane 4) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-Pax9 Antibody, clone 14D10A24 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-Pax9 Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower) as a loading control. Lane M: Molecular weight ladder.
Cat # Size Price Save
658202 100 µg ¥68,420
Description

PAX9 is a transcription factor belonging to the PAX family, which is composed of nine members defined by the presence of a paired box domain responsible for DNA binding. PAX9 is expressed at the E10 stage and downregulated at the bell stage. It is controlled by Fgf/Bmp signaling and plays an essential role in tooth development. PAX9 deficient mice fail to form teeth and show developmental arrest at the bud stage. In humans, heterozygous mutation in PAX9 is associate with tooth agenesis. These findings support the role of PAX9 in tooth development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human PAX9 recombinant protein (131-341 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody binds non-specifically to a protein over 250kDa therefore it is not recommended to use in immunofluorescence staining.

Product Citations
  1. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
RRID
AB_2562877 (BioLegend Cat. No. 658202)

Antigen Details

Structure
341 amino acids in human and 342 amino acids in mouse, with predicted molecular weight of 36 kD. Contains a paired box domain responsible for DNA binding.
Distribution

Nucleus.

Function
PAX9 is a transcription factor essential for tooth development.
Interaction
PAX9 interacts with KDM5B.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Almeida CV, et al. 2010. J. Appl. Oral Sci. 18:482.
2. Wang Y, et al. 2009. Cells Tissues Organs. 189:80.
3. Pereira TV, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:5676.
4. Mensah JK, et al. 2004. J. Biol. Chem. 279:5924.
5. Peters H, et al. 1998. Genes Dev. 12:2735.
6. Guala A, et al. 2008. Int. J. Paediatr. Dent. 18:441.

Gene ID
5083 View all products for this Gene ID
UniProt
View information about PAX9 on UniProt.org
Go To Top Version: 6    Revision Date: 04/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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