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Purified anti-pig CD4a Antibody

Pricing & Availability
Clone
74-12-4 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell surface antigen T4/Leu-3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
74-12-4_PURE_CD4a_Antibody_v2_062421.png
Porcine peripheral blood lymphocytes were stained with anti-swine CD3 Alexa Fluor® 647 and purified anti-pig CD4a (clone 74-12-4) (left), or purified mouse IgG2b, κ isotype control (right), followed by anti-mouse IgG FITC.
  • 74-12-4_PURE_CD4a_Antibody_v2_062421.png
    Porcine peripheral blood lymphocytes were stained with anti-swine CD3 Alexa Fluor® 647 and purified anti-pig CD4a (clone 74-12-4) (left), or purified mouse IgG2b, κ isotype control (right), followed by anti-mouse IgG FITC.
Cat # Size Price Save
250002 100 µg ¥45,320
Description

CD4 is a 55 kDa, type I glycoprotein expressed by tymocytes, helper T cells (Th), plasmacytoid dendritic cells (also known as natural interferon-producing cells or NIPC). In Pigs there a CD4+/CD8+ double positive subset that increase with age and have characteristics of memory T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Swine thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 74-12-4 recognizes the allele CD4.A in swine T cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Pescovitz, M.D. et al. 1984. J. Immunol. 133:368 (FC, IP)
  2. Pescovitz, M.D. et al. 1985. J. Immunol. 134:37 (FC)
  3. Saalmuller, A., et al. 1987. Eur. J. Immunol. 17:1297 (FC)
RRID
AB_2894685 (BioLegend Cat. No. 250002)

Antigen Details

Structure
55 kD, type I glycoprotein
Distribution

Thymocytes, helper T cells, plasmacytoid dendritic cells

Interaction
TCR/CD3 complex
Ligand/Receptor
MHC class II
Cell Type
T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Gustafsson K, et al. 1993. J Immunol. 151:1365-70.
  2. Blanc F, et al. 2018. Immunogenetics. 70:209-222.
  3. Zhang F, et al. 2019. Gene. 707:22-29.
Gene ID
404704 View all products for this Gene ID
UniProt
View information about CD4a on UniProt.org
Go To Top Version: 1    Revision Date: 05/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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