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Purified anti-PLK-1 Antibody

Pricing & Availability
Clone
3F8 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Isotype
Mouse IgG1, κ
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Product Citations
publications
3F8_Purified_PLK-1_Antibody_1_050318
Total lysates (15 µg protein) from 293T and 293T/PLK-1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified PLK-1 antibody, clone 3F8 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder.
  • 3F8_Purified_PLK-1_Antibody_1_050318
    Total lysates (15 µg protein) from 293T and 293T/PLK-1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified PLK-1 antibody, clone 3F8 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder.
  • 3F8_Purified_PLK-1_Antibody_2_060118
    Total lysates (15 µg protein) from HepG2, Daudi and HaCaT were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-PLK1 antibody, clone 3F8. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • 3F8_Purified_PLK-1_Antibody_3_100120
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL of Nocodazole for 20 hours were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase overnight at 4°C and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PLK-1 Phospho (Thr210) antibody (clone 2A3) for 2 hours at RT. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Purified anti-PLK-1 Antibody (Cat. No. 627702) was used as a pan PLK-1 loading control. Lane M: Molecular weight marker.
Cat # Size Price Save
627701 25 µg ¥23,320
627702 100 µg ¥50,380
Description

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino Acid: 300-603 of human PLK-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
IP, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.0 - 5.0 µg (1:100-1:500) antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed
  2. Zhu K, et al. 2022. Cell Death Differ. 2070:29. PubMed
  3. Rajamanickam S, et al. 2016. Clin Cancer Res. 22: 3524 - 3536. PubMed
  4. Auler M, et al. 2020. J Invest Dermatol. . PubMed
RRID
AB_439756 (BioLegend Cat. No. 627701)
AB_439756 (BioLegend Cat. No. 627702)

Antigen Details

Structure
Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution

Nuclear protein, highly expressed in placenta and colon

Function
Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Interaction
Interacts with nuclear distribution gene C
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.

Regulation
Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Gene ID
5347 View all products for this Gene ID
UniProt
View information about PLK-1 on UniProt.org
Go To Top Version: 4    Revision Date: 10/01/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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