Western blot of purified anti-PME-1 antibody (clone 8A6-F8) (upper). Lane 1: 15 µg of 293T cell lysate; Lane 2: 15 µg 293T/PME-1 CRISPR/Cas9 knockout (KO) cell lysate; Lane M: Molecular weight marker. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-PME-1 antibody (clone 8A6-F8) (upper). Lane 1: 15 µg of 293T cell lysate; Lane 2: 15 µg 293T/PME-1 CRISPR/Cas9 knockout (KO) cell lysate; Lane M: Molecular weight marker. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Enhanced chemiluminescence was used as the detection system.
IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded mouse pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-PME-1 antibody (clone 8A6-F8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Western blot of purified anti-PME-1 antibody (clone 8A6-F8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Protein phosphatase methylesterase-1 (PME-1) is an enzyme that catalyzes the demethylation and inactivation of protein phosphatase (PP2A). PP2A is a heterotrimeric serine/threonine phosphatase that regulates proteins of oncogenic signaling pathways such as Raf, MEK and Akt. PP2A is associated with growth inhibition and cell cycle arrest.
This monoclonal antibody was raised against a HIS-tagged, full-length mouse PME1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody is effective in immunoblotting (WB) and immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).
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