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- Clone
- 1D7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha
- Previously
-
Covance Catalog# MMS-5077
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-PP2AC (Leu309) antibody (clone 1D7). Lane 1: Molecular weight marker; Lane 2: 5 µg of human brain lysate; Lane 3: 5 µg of mouse brain lysate; Lane 4: 5 µg of SH-SY5Y cell lysate; Lane 5: 5 µg of 3T3 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-PP2AC (Leu309) antibody (clone 1D7) on formalin-fixed paraffin-embedded mouse brain tissue. The tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PP2AC (Leu309) antibody (clone 1D7) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain. When PP2A B' delta (PP2A 56 kDa regulatory subunit delta isoform) is associated with the A and C subunits, tau protein cannot bind to PP2A and be dephosphorylated, leading to increased tau aggregation.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human non-methyl Leucine 309 PP2A catalytic subunit.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution range of this reagent is 0.5 - 1 µg per ml. For immunohistochemistry, a dilution range of 0.5 - 1 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
Clone is less reactive in human samples in IHC-P applications. - RRID
-
AB_2564877 (BioLegend Cat. No. 824101)
Antigen Details
- Structure
- Expected MW: 36 kD
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Gene ID
- 5515 View all products for this Gene ID
- UniProt
- View information about PP2AC Leu309 on UniProt.org
Other Formats
View All PP2AC (Leu309) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PP2AC (Leu309) | 1D7 | WB,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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