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- Clone
- 3G4A26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- V(D)J recombination-activating protein 2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
During T cell and B cell development, RAG2 works together with RAG1 to induce DNA rearrangement and assembly of antigen receptor coding segments. Defects in RAG2 gene lead to the complete lack of mature T and B lymphocytes and result in severe combined immunodeficiency (SCID). RAG2 also functions in chromosome binding through its unique plant homeodomain (PHD). Sequestration of recombination signal sequences (RSS) within the nucleosome by RAG2 prevents access by the recombinase complex.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial RAG2 recombinant protein (70-516 aa).
- Formulation
-
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-2.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application
- RRID
-
AB_2561891 (BioLegend Cat. No. 654902)
Antigen Details
- Structure
- 527 amino acids, predicted molecular weight of 59 kD. Containing a core RAG domain with DNA cleavage activity and an atypical PHD domain responsible for binding of trimethylated histone H3 (H3K4me3) and phospholipids.
- Distribution
- Nucleus.
- Function
- RAG2 protein acts as a core subunit of the RAG complex, which is responsible for V(D)J recombination of T-cell receptors and immunoglobulins.
- Interaction
- Interacts with RAG1, HMG1, and HMG2 in V(D)J recombination protein-DNA complexes.
- Cell Type
- B cells, Tregs
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Neuroscience
- Molecular Family
- TCRs
- Antigen References
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1. Fugmann SD, et al. 2000. Annu. Rev. Immunol. 18:495.
2. Oettinger MA. 1992. Trends Genet. 8:413.
3. Hillion S, et al. 2009. Autoimmun. Rev. 8:599.
4. Deriano L, et al. 2011. Nature 471:119.
5. van Til NP, et al. 2012. Mol Ther. 20:1968.
6. Jones JM and Simkus C, et al. 2009. Arch. Immunol. Ther. Exp. 57:105. - Gene ID
- 5897 View all products for this Gene ID
- UniProt
- View information about RAG2 on UniProt.org
Other Formats
View All RAG2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-RAG2 | 3G4A26 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-RAG2
Western blot analysis of PBMC (lane 1), mouse thymus (lane 2...
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