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- Clone
- OX-41 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SIRPa, PTPNS1, BIT, MFR, p84, CD172 antigen-like family member A
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
LOU rat bone marrow cells stained with purified OX-41 (gated on granulocyte population)
Rat CD172a, also known as a signal-regulatory protein α (SIRPα), is a 75-110 kD transmembrane glycoprotein involved in the receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily primarily expressed on monocytes/macrophages, granulocytes, dendritic cells and neurons. CD172a serves as a substrate of activated receptor ttyrosine kinases (RTKs). The interaction of the CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis. CD47 (IAP) is an extracellular ligand for CD172a.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat peritoneal macrophages
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC, IP, WB, - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting3, immunoprecipitation1, and immunohistochemical staining1, 2.
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Application References
(PubMed link indicates BioLegend citation) -
- Robinson AP, et al. 1986. Immunology 57:239. (IP, IHC)
- Thai QA, et al. 1999. Stroke 30:140. (IHC)
- Adams S, et al. 1998. J. Immunol. 161:1853. (WB)
- Product Citations
-
- RRID
-
AB_2188081 (BioLegend Cat. No. 204702)
Antigen Details
- Distribution
-
Monocytes/macrophages, granulocytes, dendritic cells and neurons
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Robinson AP, et al. 1986. Immunology 57:239.
2. Thai QA, et al. 1999. Stroke 30:140.
3. Adams S, et al. 1998. J. Immunol. 161:1853. - Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Related FAQs
Other Formats
View All CD172a (SIRPα) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-rat CD172a (SIRPα) | OX-41 | FC,IHC,IP,WB |
| PE anti-rat CD172a (SIRPα) | OX-41 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat CD172a (SIRPα)
LOU rat bone marrow cells stained with purified OX-41 (gated... -
PE anti-rat CD172a (SIRPα)
LOU rat bone marrow cells stained with OX-41 PE (gated on gr...
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