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- Clone
- QA20A71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Macrosialin, GP110, LAMP4, Scavenger Receptor D1 (SR-D1)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Lew/SS rat peritoneal macrophages were fixed, permeabilized, and intracellularly stained with purified anti-rat CD68 recombinant (clone QA20A71) (left), or purified mouse IgG1, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with anti-rat CD11b/c APC.
Clone QA20A71 recombinant monoclonal antibody recognizes the rat CD68, also known as macrosialin, is a 110 kD integral membrane glycoprotein from the lysosomal-associated membrane protein (LAMP) family. It is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages, and is considered a pan-macrophage marker in the rat. CD68 may have functions relating to phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Reported Reactivity
- Bovine
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone QA20A71 can completely block the binding of parental clone to target cells. Both Cyto-Fast™ Fix/Perm Buffer Set and True-Nuclear™ Transcription Factor Buffer Set work well. This clone does not react to human and mouse.
- RRID
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AB_2936609 (BioLegend Cat. No. 201002)
Antigen Details
- Structure
- Lysosome-associated membrane glycoprotein; single-pass type I membrane protein, 110 kD
- Distribution
-
Macrophages, Langerhans cells, and at low levels on dendritic cells
- Function
- May involved in cell-cell interaction or cell-ligand interaction
- Cell Type
- Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules, Innate Immune Signaling
- Antigen References
-
- Dijkstra CD, et al. 1985. Immunology. 54:589-99.
- Damoiseaux JG, et al. 1994. Immunology. 83:140-7.
- Gene ID
- 287435 View all products for this Gene ID
- UniProt
- View information about CD68 on UniProt.org
Related FAQs
Other Formats
View All CD68 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-rat CD68 Recombinant Antibody | QA20A71 | ICFC |
| Purified anti-rat CD68 Recombinant Antibody | QA20A71 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-rat CD68 Recombinant Antibody
Lew/SS rat peritoneal macrophages were fixed, permeabilized,... -
Purified anti-rat CD68 Recombinant Antibody
Lew/SS rat peritoneal macrophages were fixed, permeabilized,...
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