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Purified anti-SARS-CoV-2 Nucleocapsid Antibody

Pricing & Availability
Clone
A20087H (See other available formats)
Regulatory Status
RUO
Other Names
Nucleocapsid phosphoprotein, N, Nucleoprotein
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20087H_PURE_SARS-CoV-2_Nucleocapsid_Antibody_1_031721.png
Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • A20087H_PURE_SARS-CoV-2_Nucleocapsid_Antibody_1_031721.png
    Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087H) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • A20087H_PURE_SARS-CoV-2_Nucleocapsid_Antibody_2_031721.png
    Recombinant nucleocapsid protein coated onto Costar™ 96-well, high binding assay plate at 2 µg/mL was incubated with a dilution series of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087H). Bound antibodies were detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
  • A20087H_PURE_SARS-CoV-2_Nucleocapsid_Antibody_3_042021.png
    His-tagged SARS-CoV-2 Nucleocapsid transfected CHO cells were stained with anti-Nucleocapsid (clone A20087H) purified (left) or mouse IgG2b, κ isotype control (clone MPC-11) purified (right) followed by anti-mouse IgG2b PE (clone RMG2b-1) and APC anti-His Tag (clone J095G46).
Cat # Size Price Save
946101 25 µg ¥24,640
946102 100 µg ¥73,700
Description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.

The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection.  N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SARS-CoV-2 Nucleocapsid recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SARS-CoV-2 is 90% homologous to SARS-CoV.  Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested.

BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H

  • Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application
  • Both clones have been verified for direct ELISA and displayed similar EC50 values for this application
  • A20087H has been verified for ICFC, while A20087F is not recommended for this application
RRID
AB_2892515 (BioLegend Cat. No. 946101)
AB_2892515 (BioLegend Cat. No. 946102)

Antigen Details

Structure
SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
Distribution

Viral nucleoprotein, host cell Golgi apparatus

Function
RNA binding, transcription regulation
Antigen References
  1. Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623.
  2. Yoshimoto FK. 2020. Protein J. 39(3):198-216.
  3. Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
Gene ID
43740575 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org
Go To Top Version: 2    Revision Date: 04/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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