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- Clone
- 1A9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SH2 Domain Containing 1A, SAP, T-Cell Signal Transduction Molecule SAP, SLAM-Associated Protein, T Cell Signal Transduction Molecule SAP, MTCP1, XLPD
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell lysates (15 µg protein) from Jurkat (lane 1), mouse thymus (lane 2) and HeLa (lane 3, negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-SH2D1A (clone 1A9) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
SH2D1A, also known as SAP, is a small adaptor protein which plays a major role in the bidirectional stimulation of T and B cells. SH2D1A is predominantly expressed in T cells and NK cells, and functions to regulate signal transduction pathways downstream of the SLAM family of surface receptors. The SH2D1A gene is frequently mutated in patients with X-linked lymphoproliferative disease. Malfunction of the SLAM-SAP pathway results in autoimmune diseases, tumors of the lymphoid tissues and inadequate responses to infectious agents.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Full length purified human SH2D1A.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2629824 (BioLegend Cat. No. 690702)
Antigen Details
- Structure
- 128 amino acids with a characteristic of SH2 domain. The predicted molecular weight is approximately 14kD.
- Distribution
- T cells, NK cells and monocytes.
- Function
- Stimulation of T and B cells.
- Interaction
- SLAM family, SH2D1B, and FYN.
- Ligand/Receptor
- SLAM family; SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7.
- Cell Type
- T cells, NK cells, Monocytes
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
-
1. Tangye SG. 2014. J. Clin. Immunol. 34:772.
2. Yang X, et al. 2012. Pediatr. Int. 54:447.
3. Ma CS, Deenick EK. 2011. Ann. N Y Acad. Sci. 1217:32-44.
4. Detre C, et al. 2010. Semin. Immunopathol. 32:157.
5. Vilar ML, et al. 2011. Braz. J. Med. Biol. Res. 44:276. - Gene ID
- 4068 View all products for this Gene ID
- UniProt
- View information about SH2D1A on UniProt.org
Other Formats
View All SH2D1A (SAP) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SH2D1A (SAP) | 1A9 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-SH2D1A (SAP)
Total cell lysates (15 µg protein) from Jurkat (lane 1...
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