- Clone
- W17121A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NAD-dependent protein deacetylase sirtuin-7, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT2L7, Sirtuin7, sirtuin-7, sirtuin 7
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W17121A_PURE_SIRT7_Antibody_1_072418 W17121A_PURE_SIRT7_Antibody_1_072418](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W17121A_PURE_SIRT7_Antibody_1_072418_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg protein) from HeLa, Jurkat, HEK293 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:1000 (0.5 µg/mL) diluted purified anti-SIRT7 antibody (clone W17121A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control. Lane M is the MW ladder. -
Total cell lysate (400 µg protein) from HeLa cells were incubated with 2.5 µg rat IgG2a isotype control (lane 1) or anti-SIRT7 antibody (lane 2) at 4°C for 3 hours, followed by incubating with the Protein-G beads overnight. Immunoprecipitates and total protein (10% of input, lane 3) were eluted with SDS loading buffer and resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-SIRT7 antibody (clone W17121A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Lane M is the MW ladder. IgGHC: IgG heavy chain, IgGLC: IgG light chain.
Cat # | Size | Price | Save |
---|---|---|---|
666101 | 25 µg | ¥22,220 | |
666102 | 100 µg | ¥55,660 |
Sirtuins are highly conserved NAD+-dependent deacetylases and function in various biological processes, including metabolism, stress response, senescence, genomic stability, and tumorigenesis. SIRT7 is the only sirtuin predominantly localized in the nucleoli and binds rDNA regulatory sequences. In addition to the function in activating transcription of ribosomal genes (rDNA), SIRT7 has been found to deacetylate histone H3K18Ac and maintain the deacetylated stat. SIRT7 plays a role in cell viability by promoting cell cycle progression and proliferation, as well as inhibiting apoptosis. Misregulated expression of SIRT7 has been linked to the progression of various cancers. SIRT7-deficient mice show shortened lifespan and develop aging related pathologies.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human SIRT7 recombinant protein (1-250 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:250-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 2.5 µg per 400 µg total protein. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone can not be used for ICC application.
- RRID
-
AB_2749942 (BioLegend Cat. No. 666101)
AB_2749943 (BioLegend Cat. No. 666102)
Antigen Details
- Structure
- 400 amino acids with a predicted molecular weight of 45 kD. Contains a sirtuin-type deacetylase domain.
- Distribution
-
Nucleolus
- Function
- SIRT7 is a histone deacetylase involved in the regulation of cell cycle progression, DNA repair, againg, stress resistance, rDNA transcription, chromatin remodeling, lipid metabolism, and tumorigenesis.
- Interaction
- Interacts with UBTF, MYBBP1A, SMARCA5/SNF2H, BAZ1B/WSTF, ELK4, histone H2A, and histone H2B.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics
- Antigen References
-
- Fang J, et al. 2017. Proc. Natl. Acad. Sci. U S A. 114:E8352.
- Tang X, et al. 2017. Nat. Commun. 8:318.
- Yu J, et al. 2017. Cell. Rep. 18:1229.
- Chen S, et al. 2016. Nat. Commun. 7:10734.
- Zhang PY, et al. 2016. Nucleic Acids Res. 44:3629.
- Yu H, et al. 2014. Clin. Cancer Res. 20:3434.
- Gene ID
- 51547 View all products for this Gene ID
- UniProt
- View information about SIRT7 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All SIRT7 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SIRT7 | W17121A | WB,IP |
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