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Purified anti-SKIL (SnoN) Antibody

Pricing & Availability
Clone
O91H2 (See other available formats)
Regulatory Status
RUO
Other Names
SnoN
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_O91H2_Purified_SKIL_Antibody_031519.png
Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SKIL (SnoN) Antibody, clone O91H2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_O91H2_Purified_SKIL_Antibody_031519.png
    Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SKIL (SnoN) Antibody, clone O91H2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • O91H2_PURE_SKIL_Antibody_050418
    Total cell lysates (15 µg protein) from HEK293T (negative control), NTERA-2, and HaCaT cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SKIL (SnoN) antibody. Proteins were visualized by chemiluminescence detection using goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: MW ladder.
Cat # Size Price Save
644201 25 µg ¥22,220
644202 100 µg ¥55,660
Description

Ski-like proto oncogene (SKIL) is a transcriptional repressor in the SMAD-TGF-β signaling axis that plays a critical role in TGF-β activation. It functions as a key regulator of proliferation and differentiation. Upon activation of TGFβ signaling, SMAD3 enters the nucleus and facilitates the ubiquitination and subsequent degradation of SKIL by the anaphase- promoting complex, resulting in derepression of SKIL-regulated genes and enhanced BMP-SMAD signaling. Additionally, SKIL is targeted for degradation by the E3 ligase Arkadia. The region corresponding to amino acids 1-96 of SKIL mediates its interaction with SMAD3, with residues 89-92 playing a critical role in stabilizing the interaction. The protein also contains a D-box motif between residues 162-174 that facilitates its interaction with other ubiquitin-conjugating enzyme complexes. Lysines 440, 446, and 449 are required for efficient ubiquitination upon TGF-β-activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human SKIL recombinant protein (307-684) produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2715795 (BioLegend Cat. No. 644201)
AB_2715795 (BioLegend Cat. No. 644202)

Antigen Details

Structure
SKIL/SnoN is a 684 amino acid protein with a predicted molecular weight of 76.9 kDa.
Distribution

Nucleus

Function
SKIL functions as a transcriptional repressor when bound to the regulatory promoter region of TGFβ-responsive genes.
Interaction
SMAD2, SMAD3, RNF111, APC1, WWP1, RNF168, SMURF2
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Yin X, et al. 2016. Oncol Rep. 36:1535.
2. Caligaris C, et al. 2015. Biochim. Biophys. Acta. 1850(9):1832.
3. Annala M, et al. 2015. Oncotarget. 6:6235.
4. Ikeuchi Y, et al. 2014. J. Biol. Chem. 289: 25067.
5. Hagerstrand D, et al. 2013. Cancer Discover. 3: 1044.
6. Tsuneyoshi N, et al. 2012. Genes Dev. 26: 2471.
7. Aarntzen EH, et al. 2012. Cancer Res. 72: 6102.
8. Zhang L, et al. 2011. J. Biol. Chem. 286: 10041.
9. Stroschein SL, et al. 2001. Genes Dev. 15: 2822. 

Gene ID
6498 View all products for this Gene ID
UniProt
View information about SKIL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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