- Clone
- 6H5-E3-C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SMAD family member 2, CHTD8, hMAD-2, JV18-1, LDS6, MADH2, MADR2, HSMAD2MAD Homolog 2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
606451 | 25 µg | ¥25,960 | |
606452 | 100 µg | ¥88,000 |
SMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-B receptor. SMAD2 gets phosphorylated by the TGF-B receptor in response to TGF-B signal, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2/SMAD4 protein participates in gene regulation by forming a transcription repressor complex in association with other cofactors.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragments of human SMAD2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Since SMAD2 shares a high percentage of identity with SMAD3, this clone recognizes both SMAD2 and SMAD3, which can be resolved by Western Blot.
- RRID
-
AB_2924617 (BioLegend Cat. No. 606451)
AB_2924617 (BioLegend Cat. No. 606452)
Antigen Details
- Structure
- SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD. SMAD2 is very similar in sequence and structure to SMAD3.
- Distribution
-
Non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-B receptor. Phosphorylated SMAD2 is present in the cellular nucleus bind to target promotor sequences.
- Function
- The function of SMAD2 is mainly as a gene expression regulator. SMAD2 acts as a sequence specific transcriptional repressor.
- Interaction
- Non-phosphorylated SMAD2 interacts with SARA (SMAD anchor for receptor activation) protein. Phosphorylated SMAD2 interacts with SMAD4 and other members of the transcription repressor complex.
- Ligand/Receptor
- SMAD2 is part of the TGF-B signaling pathway but it is not a ligand/receptor protein
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Gene ID
- 4087 View all products for this Gene ID
- UniProt
- View information about SMAD2 on UniProt.org
Related FAQs
Other Formats
View All SMAD2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SMAD2 | 6H5-E3-C11 | WB |
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Purified anti-SMAD2
Whole cell extracts (15 µg total protein) from the indicated...
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