- Clone
- W21150C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MAD homolog 2, JV18-1, MAD-2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
624851 | 25 µg | ¥36,300 | |
624852 | 100 µg | ¥91,300 |
SMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-β receptor. SMAD2 gets phosphorylated by the TGF-β receptor in response to TGF-β signaling, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2/SMAD4 protein complex participates in gene regulation by forming a transcription repressor complex in association with other cofactors.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human SMAD2 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.016 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone cross-reacts with mouse.
For intracellular flow cytometric staining with this clone, we recommended using Fixation Buffer (Cat. No. 420801), True-Phos™ Perm Buffer (Cat. No. 425401), Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not applicable.
We do not recommend this product for use in immunocytochemistry (ICC). - RRID
-
AB_3097247 (BioLegend Cat. No. 624851)
AB_3097247 (BioLegend Cat. No. 624852)
Antigen Details
- Structure
- SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD
- Distribution
-
Skeletal muscle, endothelial cells, heart and placenta; non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-β receptor; phosphorylated SMAD2 is present in the cellular nucleus and binds to target promoter sequences.
- Function
- Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Interaction
- TAZ/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-β, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1
- Ligand/Receptor
- SMAD2 is part of the TGF-β signaling pathway but it is not a ligand/receptor protein
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Nakao A, et al. 1997. EMBO J. 16:5353–62.
- Attisano L. and Wrana JL, et al. 2002. Science. 296:1646-47.
- Moustakas A. et al. 2001. J Cell Sci. 114: 4359-69.
- Gene ID
- 4087 View all products for this Gene ID
- UniProt
- View information about SMAD2 on UniProt.org
Related FAQs
Other Formats
View All SMAD2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-SMAD2 | W21150C | WB,ICFC |
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