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Purified anti-SMARCA4/BRG1 Antibody

Pricing & Availability
Clone
W22111B (See other available formats)
Regulatory Status
RUO
Other Names
BRG1, BAF190, SNF2, SWI2, SNF2L4, SNF2b, BAF190A, SMCA4, Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A (BAF190A), Mitotic growth and transcription activator, BRG-1, Protein brahma homolog 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22111B_PURE_SMARCA4-BRG1_Antibody_1_022224
IHC staining of SMARCA4/BRG1 on formalin-fixed paraffin-embedded human colon tissue, following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502). Tissue sections were incubated without (panel A) and with (panel B and C) Purified anti-SMARCA4/ BRG1 (clone W22111B) overnight at 4°C followed by incubation with Alexa Fluor® 647 Goat anti-Rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Panel B is overlay of DAPI and Alexa Fluor® 647 channel, and panel C is Alexa Fluor® 647 channel only. Images were captured using a 40X objective.
  • W22111B_PURE_SMARCA4-BRG1_Antibody_1_022224
    IHC staining of SMARCA4/BRG1 on formalin-fixed paraffin-embedded human colon tissue, following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502). Tissue sections were incubated without (panel A) and with (panel B and C) Purified anti-SMARCA4/ BRG1 (clone W22111B) overnight at 4°C followed by incubation with Alexa Fluor® 647 Goat anti-Rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Panel B is overlay of DAPI and Alexa Fluor® 647 channel, and panel C is Alexa Fluor® 647 channel only. Images were captured using a 40X objective.
  • W22111B_PURE_SMARCA4-BRG1_Antibody_2_022224
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SMARCA4/BRG1 (clone W22111B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-Rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • W22111B_PURE_SMARCA4-BRG1_Antibody_3_022224
    A-549 cells (low expression control, open histogram), and MCF-7 cells (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-SMARCA4/BRG1 (clone W22111B) or Purified Rat IgG2a, κ Isotype Control (control, open histogram dashed line) (representative for both untreated or treated cells) (Cat. No. 400501) followed by Alexa Fluor® 647 Goat anti-Rat IgG (Cat. No. 405416).
Cat # Size Price Save
632451 25 µg ¥35,200
632452 100 µg ¥95,700
Description

The transcription regulator SMARCA4/BRG1 is part of the SWI/SNF protein family and regulates chromatin structure in an ATP-dependent manner. It has helicase and ATPase activities which are required for chromatin topology remodeling. SMARCA4/BRG1 is important in transcriptional regulation of several genes involved in stress responses, neurogenesis, cell cycle, and differentiation. In this context, transcription of c-FOS in resting neurons is inhibited by recruitment of phospho RB1-HDAC complex by SMARCA4/BRG1. Alternatively, the CREST-BRG1 complex induces NR2B expression by binding to its promotor. In addition, SMARCA4/BRG1 regulates the expression of the tumorigenic protein CD44. Lastly, mutations in the SMARCA4 gene have been associated with rhabdoid tumor predisposition syndrome type 2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse recombinant protein fragment encompassing amino acids 217-294
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 5 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097587 (BioLegend Cat. No. 632451)
AB_3097587 (BioLegend Cat. No. 632452)

Antigen Details

Structure
SMARCA4/BRG1 is a 1647 amino acids length protein with a molecular weight of 185 kD.
Distribution

Localized to the nucleus (nucleoplasm, nucleoli fibrillar center, nucleoli rim)

Function
Alter DNA-nucleosome topology by remodeling chromatin; Chromatin regulator, Helicase, Hydrolase
Ligand/Receptor
ATP binding, Nucleotide binding
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References
  1. Mardinian K, et al. 2021. Mol Cancer Ther. 20:2341-2351.
  2. Peng L, et al. 2021. Front Immunol. 12:762598.
  3. Xue Y, et al. 2021. Nat Commun. 12:5404.
  4. Yoshikawa T, et al. 2021. J Pathol. 255:257-269.
Gene ID
6597 View all products for this Gene ID
UniProt
View information about SMARCA4/BRG1 on UniProt.org
Go To Top Version: 1    Revision Date: 02/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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