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- Clone
- 2G9.F10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule-associated protein tau, paired helical filament tau, PHF tau, neurofibrillary tangle protein
- Previously
-
Covance Catalog# MMS-5092
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Tau, 157-168 antibody (clone 2G9.F10). Lane 1: Molecular weight marker; Lane 2: 30 µg of normal human brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Tau, 157-168 antibody (clone 2G9.F10) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Save |
|---|---|---|---|
| 824602 | 25 µL | ¥24,640 | |
| 824601 | 100 µL | ¥60,500 |
Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.
Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation. Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains. Three isoforms have three binding domains and the remaining three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule). Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence corresponding to amino acids 157-168 of human tau protein, and conjugated to KLH via an N-terminal Cysteine residue.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
- RRID
-
AB_2564882 (BioLegend Cat. No. 824602)
AB_2564882 (BioLegend Cat. No. 824601)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Tau
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All Tau Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tau, 157-168 | 2G9.F10 | IHC-P,WB |
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