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Purified anti-TDP43 Phospho (Ser409/410) Antibody (Previously Covance catalog# SIG-39852)

Pricing & Availability
Clone
1D3/TDP-43 (See other available formats)
Regulatory Status
RUO
Other Names
TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
Previously
Covance Catalog# SIG-39852
Isotype
Rat IgG2a
Ave. Rating
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Product Citations
publications
Description

TDP43 (aka TARDBP) was originally identified as a protein which binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins which bind single stranded RNA. TDP-43 localizes to the nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.  Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. Hyperphosphorylated, ubiquitinated, and cleaved in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This antibody was raised against a phosphopeptide corresponding to: i) the C-terminal domain of human TDP43; and ii) inclusive of phosphorylated serine residues 409 and 410.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC, ICC, ELISA- Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000
 

Application Notes

This antibody is effective in immunoblotting (WB)

Positive Control: Rat Brain nuclear lysate
Expected MW: ~43 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Neumann M, et al. 2009. Acta Neuropathol. 117:137-49.
  2. Sarroukh R, et al. 2011. Cell Mol Life Sci. 68:1429. (WB, IHC, ELISA, IF)
  3. Ramakrishnan M, et al. 2009. Biochemistry 48:10405. (WB)
  4. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:10323. (WB, IHC)
  5. Diomede L, et al. 2014. Neurobiol Dis. 62:521. (IHC)
Product Citations
  1. Melikyan ZA, et al. 2022. Neurobiol Aging. 116:12. PubMed
  2. Ackermans NL, et al. 2022. Acta Neuropathol. 144:5. PubMed
  3. Latimer CS, et al. 2022. Methods Mol Biol. 2561:3. PubMed
  4. Keating SS, et al. 2023. Cell Mol Life Sci. 80:95. PubMed
  5. Gasset-Rosa F, et al. 2019. Neuron. 102:339. PubMed
  6. Gleixner AM, et al. 2022. Nat Commun. 13:3380. PubMed
  7. Liu EA, et al. 2021. Neuropathol Appl Neurobiol. 47:1019. PubMed
  8. Wiesner D, et al. 2017. Exp Neurol. 10.1016/j.expneurol.2017.09.011. PubMed
  9. Wani A, et al. 2021. Cell Reports. 36(3):109399. PubMed

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, TDP43
Gene ID
23435 View all products for this Gene ID 298648 View all products for this Gene ID
UniProt
View information about TDP43 Phospho Ser409/410 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-TDP43 Phospho (Ser409/410)

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